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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
monoclonal
species reactivity
chicken
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NCAN(1463)
General description
Neurocan is the major soluble chondroitin sulfate proteoglycan in the brain. It is thought to play a functional role in axonal growth and guidance and in the establishment of specific neural pathways during embryonic brain development. Neurocan expression in the brain is developmentally regulated. Early on the major form of neurocan consists of a 245 kD core protein with approximately two chondroitin sulfate glycosoaminoglycan chains of 22 kD each. Later neurocan comprises a 180 kD core protein. Both forms of neurocan contain only chondroitin 4-sulfate glycosoaminoglycan chains. By virtue of their high expression at sites of neurnal damage and trauma, chondroitin sulfate proteoglycans, including neurocan, are thought to inhibit successful nerve regeneration.
Specificity
Neurocan, chicken. Recognizes the middle region of neurocan.
Application
Anti-Neurocan Antibody is an antibody against Neurocan for use in ELISA, IP & WB.
Western blot: <0.5 μg/mL
Western blot using anti-chick Neurocan (MAB5234). Samples are 1) Untreated embryonic chick brain extract, 2) chondroitinase-treated embryonic chick brain extract, 3) GST fusion proteins from the middle region of chick neurocan.
Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.
Immunoprecipitation: 1 μg/mL
ELISA: 1 μg/mL, excellent for core protein, good for monomer
Immunocytochemistry: not tested
Immunohistochemistry: does not work on fixed samples, unfixed has not been tested.
Optimal working dilutions must be determined by the end user.
Western blot using anti-chick Neurocan (MAB5234). Samples are 1) Untreated embryonic chick brain extract, 2) chondroitinase-treated embryonic chick brain extract, 3) GST fusion proteins from the middle region of chick neurocan.
Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.
Immunoprecipitation: 1 μg/mL
ELISA: 1 μg/mL, excellent for core protein, good for monomer
Immunocytochemistry: not tested
Immunohistochemistry: does not work on fixed samples, unfixed has not been tested.
Optimal working dilutions must be determined by the end user.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Brennaman, LH; Zhang, X; Guan, H; Triplett, JW; Brown, A; Demyanenko, GP; Manis et al.
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Rowena C Cua et al.
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Acute trauma to the central nervous system (CNS) can result in permanent damage and loss of function related to the poor regeneration of injured axons. Injured axons encounter several barriers to regeneration, such as the glial scar at the injury
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Scientific reports, 9(1), 13634-13634 (2019-09-22)
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