All Photos(5)

MAB4381

Sigma-Aldrich

Anti-TRA-1-81 Antibody, clone TRA-1-81

clone TRA-1-81, Chemicon®, from mouse

eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

saturated ammonium sulfate (SAS) precipitated

antibody product type

primary antibodies

clone

TRA-1-81, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

input

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

isotype

IgM

shipped in

wet ice

General description

Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). Embryonal carcinoma, as do all human pluripotent stem cells, expresses TRA-1-60 and TRA-1-81 antigens, and although their molecular identities are unknown, they are commonly used as markers of undifferentiated pluripotent human stem cells.

Specificity

This antibody reacts with TRA-1-81 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-81 and TRA-1-60 monoclonal antibodies (MAB4360) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.

Immunogen

Human embryonal carcinoma cell line 2102Ep

Application

Immunofluorescence: A previous lot of this antibody was used in IF.

Western Blot: A previous lot of this antibody was used in WB.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunohistochemistry: A previous lot of this antibody was used in IH.

Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Pluripotent & Early Differentiation
Anti-TRA-1-81 Antibody, clone TRA-1-81 detects level of TRA-1-81 & has been published & validated for use in WB, FC, IF, IP & IC.
Research Category
Stem Cell Research

Target description

420/250 kDa

Physical form

Liquid in 0.05 M Potassium Phosphate, 0.3 M NaCl, pH 8.0 with 0.05% Sodium Azide.
Format: Purified
Ammonium Sulfate Precipitation

Storage and Stability

Stable for 1 year at from date of receipt.

Analysis Note

Control
Human embryonic stem cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

James A Byrne et al.
PloS one, 4(9), e7118-e7118 (2009-09-24)
The derivation of induced pluripotent stem cells (iPSCs) provides new possibilities for basic research and novel cell-based therapies. Limitations, however, include our current lack of understanding regarding the underlying mechanisms and the inefficiency of reprogramming. Here, we report identification and
Human embryonic stem cell lines derived from single blastomeres.
Irina Klimanskaya, Young Chung, Sandy Becker, Shi-Jiang Lu, Robert Lanza
Nature null
Sequential genetic modification of the hprt locus in human ESCs combining gene targeting and recombinase-mediated cassette exchange.
Alexandra I Di Domenico, Ioannis Christodoulou, Steve C Pells, Jim McWhir, Alison J Thomson
Cloning and Stem Cells null
Kenta Nakamura et al.
PloS one, 4(11), e7780-e7780 (2009-11-26)
Reprogramming adult human somatic cells to create human induced pluripotent stem (hiPS) cell colonies involves a dramatic morphological and organizational transition. These colonies are morphologically indistinguishable from those of pluripotent human embryonic stem (hES) cells. G protein-coupled receptors (GPCRs) are
Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells.
Raya, Angel, et al.
Nature, 460, 53-59 (2009)

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