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MAB3516

Sigma-Aldrich

Anti-Heat Shock Protein 70 Antibody, clone W27

clone W27, Chemicon®, from mouse

Synonym(s):

HSP70

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

W27, monoclonal

species reactivity

bovine, human, monkey

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_002154.3
NM_198431.1

UniProt accession no.

P34932

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HSPA4(3308)

Specificity

Reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70.

POSITIVE CONTROL:

HeLa cells, BT474 cells or breast carcinoma.

Immunogen

HSP70 from HeLa cells.

Application

Immunoblotting: 0.5-1 μg/mL

Immunohistochemistry (frozen and formalin/paraffin): 2-4 μg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Immunoprecpitation: 2 μg/mg protein lysate (use Protein G)

Flow cytometry

Optimal working dilutions must be determined by end user.
This Anti-Heat Shock Protein 70 Antibody, clone W27 is validated for use in IP, WB, IH(P) for the detection of Heat Shock Protein 70.

Physical form

Format: Purified

Analysis Note

Control
POSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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