All Photos(7)
MAB3418
Anti-MAP2 Antibody, clone AP20
clone AP20, Chemicon®, from mouse
All Photos(7)
Synonym(s):
Microtubule Associated Protein, MAP2A and 2B
eCl@ss:
32160702
NACRES:
NA.41
Quality Level
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AP20, monoclonal
species reactivity
mouse, rat, quail, Xenopus, chicken, bovine, human
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
Gene Information
chicken ... Map2(424001)
frog ... Map2(780043)
human ... MAP2(4133)
mouse ... Map2(17756) , Map2(281294)
rat ... Map2(25595)
General description
MAP-2 (microtubule associated protein-2) is one of several high molecular weight proteins that play an important role in brain neuron microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2. MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds. (Caceres, 1986; Dotti, 1987).
Specificity
MAB3418 binds specifically to MAP-2.
Expected to cross-react with human, cow, chicken, quail, and Xenopus.
Immunogen
Bovine brain microtubule protein.
Application
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
Anti-MAP2 Antibody, clone AP20 detects level of MAP2 & has been published & validated for use in IH & WB.
Western Blot:
60 ng/mL. In SDS-PAGE, MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 280-300 kD representing the MAP-2A and -2B isoforms but not the 70 kDa MAP-2C isoform. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Users should run 4%-20% SDS-PAGE gradient gels.
Immunohistochemistry:
5 µg/mL (4% PFA fixed frozen sections).
Immunohistochemistry:
5 µg/mL of a previous lot was used. Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.
Optimal working concentrations must be determined by the end user.
60 ng/mL. In SDS-PAGE, MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 280-300 kD representing the MAP-2A and -2B isoforms but not the 70 kDa MAP-2C isoform. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Users should run 4%-20% SDS-PAGE gradient gels.
Immunohistochemistry:
5 µg/mL (4% PFA fixed frozen sections).
Immunohistochemistry:
5 µg/mL of a previous lot was used. Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.
Optimal working concentrations must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Quality
Routinely evaluated by Western Blot on Rat Brain lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected MAP2 on 10 μg of Rat Brain lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected MAP2 on 10 μg of Rat Brain lysate.
Target description
230 kDa
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, pH 7.6, 0.25 M NaCl with 0.1% sodium azide.
Storage and Stability
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Analysis Note
Control
Brain, Neuronal culture, Human glioblastoma T98G cells.
Brain, Neuronal culture, Human glioblastoma T98G cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificate of Analysis
Enter Lot Number to search for Certificate of Analysis (COA).
Certificate of Quality
Enter Lot Number to search for Certificate of Quality (COQ).
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