MAB3234
Anti-Cytokeratin 18 Antibody, clone RGE53
clone RGE53, Chemicon®, from mouse
About This Item
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RGE53, monoclonal
species reactivity
human, pig, mouse, chicken, canine, rat, rabbit, hamster
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
suitability
not suitable for immunohistochemistry (Paraffin)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... KRT18(3875)
General description
Specificity
Immunogen
Application
Cell Structure
Cytokeratins
Immunohistochemistry on fresh frozen or acetone fixed tissue sections. Formalin fixed, paraffin embedded tissues are not recommended for RGE53.
Immunocytochemistry: (10μg/mL) Light fixation with 4% formalin, 2.5% PFA ( 5-10 minutes room temperature) or acetone. {See, Barrett, et al 2001} Permeabilization with 0.2% triton X-100 in blocking buffer if using formalin or PFA fixations.
Flow cytometry: Acetone fixed/ethanol fixed cells {See Mezosi, E et al, 2002}.
Optimal working dilutions must be determined by the end user.
Target description
Linkage
Physical form
Storage and Stability
Analysis Note
Skin , Pancreas/tumor
Other Notes
Legal Information
Disclaimer
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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