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MAB318

Sigma-Aldrich

Anti-Tyrosine Hydroxylase Antibody, clone LNC1

ascites fluid, clone LNC1, Chemicon®

Synonym(s):
TH, Tyrosine Monooxygenase
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

LNC1, monoclonal

species reactivity

chicken, monkey, frog, mouse, vole, human, lizard, rat, zebrafish

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... TH(7054)
rat ... Th(25085)

General description

Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Specificity

Recognizes an epitope on the outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT).

Immunogen

Tyrosine Hydroxylase purified from PC12 cells

Application

Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers
Immunohistochemistry(paraffin):
A 1:200-1:400 dilution of a previous lot was used in IH. 4% PFA fixed, frozen sections; 4% PFA fixed, paraffin sections 1:100 (Barrachina, M. et al., 2003). For paraffin sections Barrachina reported successful staining with microwave citrate acid antigen recovery, however other methods can likely be used as well.

Immunoprecipitation:
A previous lot of this antibody was used in IP (Perez, 2002).

Optimal working dilutions and protocols must be determined by end user.
Research Category
Neuroscience
Detect Tyrosine Hydroxylase using this Anti-Tyrosine Hydroxylase Antibody, clone LNC1 validated for use in IH, IH(P), IP & WB with more than 85 product citations.

Quality

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western blot:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of Mouse Brain lysates.

Target description

59-63 kDa

Physical form

Unpurified
Ascites mouse monoclonal IgG1κ fluid containing 3% BSA. Contains no preservative.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Human brain tissue, extracts from 3T3 cells treated with Forskolin (40 nM, 30 min).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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