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Key Documents

MAB3136

Sigma-Aldrich

Anti-Na+/H+ Exchanger-3 Antibody, isoform NHE3, clone 4F5

clone 4F5, Chemicon®, from mouse

Synonym(s):

NHE3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4F5, monoclonal

species reactivity

rat, rabbit

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001082697.1

UniProt accession no.

P26432

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

rabbit ... Slc9A3(100009430)
rat ... Slc9A3(24784)

Specificity

Na+/H+ exchanger, isoform NHE3.

Immunogen

Epitope: isoform NHE3
Maltose Binding Protein fusion protein containing C-terminal 131 amino acids of rabbit NHE3.

Application

Anti-Na+/H+ Exchanger-3 Antibody, isoform NHE3, clone 4F5 detects level of Na+/H+ Exchanger-3 & has been published & validated for use in IC, IH(P), IP & WB.
Immunoblotting (rabbit 80 kDa and rat ~83 kDa)

Immunohistochemistry (rat) on PLP or paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections.

Immunocytochemistry

Immunoprecipitation (use non-ionic detergent)

Optimal working dilutions must be determined by the user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Linkage

Replaces: MAB3134

Physical form

Format: Purified
Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Distribution of pH regulators in the rat laryngeal nerve: the spatial relationship between Na(+)/HCO(3)(-) cotransporters and Na(+)/H(+) exchanger type 3.
Yoshio Yamamoto, Kazuyuki Taniguchi
Neuroscience Letters null
Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney.
Biemesderfer, D, et al.
The American Journal of Physiology, 273, F289-F299 (1997)
Yanling Yan et al.
Molecular and cellular biochemistry, 367(1-2), 175-183 (2012-05-24)
We have demonstrated that ouabain regulates protein trafficking of the Na/K-ATPase α1 subunit and NHE3 (Na/H exchanger, isoform 3) via ouabain-activated Na/K-ATPase signaling in porcine LLC-PK1 cells. To investigate whether this mechanism is species-specific, ouabain-induced regulation of the α1 subunit
Lactobacillus acidophilus upregulates intestinal NHE3 expression and function.
Singh, V; Raheja, G; Borthakur, A; Kumar, A; Gill, RK; Alakkam, A; Malakooti, J; Dudeja, PK
American Journal of Physiology: Gastrointestinal and Liver Physiology null
Expression and localization of the Na+/H+ exchanger isoform NHE3 in the rat efferent ducts.
Kari M Kaunisto, Hannu J Rajaniemi
Journal of Andrology null
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