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Key Documents

MAB1999

Sigma-Aldrich

Anti-Integrin α5β1 Antibody, clone HA5

clone HA5, Chemicon®, from mouse

Synonym(s):

VLA-5

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HA5, monoclonal

species reactivity

pig, human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2bκ

NCBI accession no.

NM_002205.2

UniProt accession no.

P08648

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGA5(3678)

Related Categories

Specificity

Reacts with the fibronectin receptor VLA-5 (alpha-5beta-1) integrin complex.

Application

Anti-Integrin α5β1 Antibody, clone HA5 detects level of Integrin α5β1 & has been published & validated for use in FC & IP.
Immunoprecipitation of VLA-5 integrins from lysate of 10E6 cell equivalent requires 2 μg of MAB1999.

Flow cytometry: 10 μg/mL

Does not detect VLA-5 by Western blot.

MAB1999 does not affect VLA-5 mediated cell adhesion to fibronectin.

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified
Purified immunoglobulin from Protein A chromatography. Liquid in 0.02 M PB pH 7.6, 0.25 M NaCl, 0.1% sodium azide.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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This study identifies and characterizes the antigen recognized by monoclonal antibody (mAb) 14C5. We compared the expression of antigen 14C5 with the expression of eight integrin subunits (alpha1, alpha2, alpha3, alphav, beta1, beta2, beta3, and beta4) and three integrin heterodimers
Regulation of the MDR1 gene by transcriptional repressors selected using peptide combinatorial libraries.
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