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MAB1934

Sigma-Aldrich

Anti-Fibronectin Antibody, cell binding peptide, clone 784A2A6

purified antibody, clone 784A2A6, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

784A2A6, monoclonal

species reactivity

human, chicken

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

chicken ... Fn1(396133)
human ... FN1(2335)

Specificity

Reacts with the 11kd cell adhesive fragment and weakly inhibits cell binding to fibronectin (fibronectin mediated cell adhesion).

Immunogen

Epitope: cell binding peptide
Human plasma fibronectin.

Application

Anti-Fibronectin Antibody, cell binding peptide, clone 784A2A6 detects level of Fibronectin & has been published & validated for use in ELISA & WB.
ELISA

Microscopy

Western blotting at 1-5 μg/mL

Cellular Adhesion Studies

Note: Dilution without addition of carrier protein may cause denautration or adherence of the antibody to the vial. Dilution into a 1% bovine serum albumin containing buffer is recommended.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Physical form

Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C for up to 6 months in undiluted aliquots

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Modulating cell adhesion and spreading by control of FnIII7-10 orientation on charged self-assembled monolayers (SAMs) of alkanethiolates.
Hua Wang, Yi He, Buddy D Ratner, Shaoyi Jiang
Journal of Biomedical Materials Research Part A null
Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
Tejada, ML; Yu, L; Dong, J; Jung, K; Meng, G; Peale, FV; Frantz, GD; Hall, L; Liang et al.
Clinical cancer research : an official journal of the American Association for Cancer Research null
Hiroshi Fujii et al.
Kidney international, 64(5), 1662-1670 (2003-10-09)
One of the crucial events in lupus nephritis is the glomerular deposition of immunoglobulins (Igs), of which pathogenic properties have been proposed mostly to be either type IIor type III allergic reactions. Some of IgG3-producing hybridoma clones established from an
Karine Vallières et al.
Macromolecular bioscience, 7(5), 738-745 (2007-04-26)
Surface coating of synthetic materials is often considered to improve biomedical devices biocompatibility. In this study, we covalently bound fibronectin (FN) onto ammonia plasma-treated PTFE via two crosslinkers, namely glutaric anhydride (GA) and sulfosuccinimidyl-4-(p-maleimidophenyl)butyrate (sulfo-SMPB). With respect to clean PTFE
Souhaila Ghadhab et al.
Journal of biomedical materials research. Part A, 109(11), 2187-2198 (2021-05-02)
Intraosseous transcutaneous amputation prosthesis is a new approach in orthopedic implants that overcomes socket prosthesis problems. Its long-term performance requires a tight skin-implant seal to prevent infections. In this study, fibronectin (Fn), a widely used adhesion protein, was adsorbed or

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