MAB19101
Anti-Tenascin Antibody, clone DB7
clone DB7, Chemicon®, from mouse
Synonym(s):
Tenascin-C
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
DB7, monoclonal
species reactivity
human, rabbit, guinea pig
should not react with
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG2a
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TNC(3371)
Specificity
The antibody recognizes the Mr 250000 and 180000 tenascin polypeptides in immunoprecipitation and immunoblots the Mr 250000 polypeptide. The antibody reacts with the fibrinogen-like knob-domain of tenascin protein
Immunogen
Tenascin polypeptides isolated from spent culture supernatant of human fibroblasts isolated by affinity chromatography.
Application
Detect Tenascin using this Anti-Tenascin Antibody, clone DB7 validated for use in IF, WB, IH(P).
Immunofluorescence: 1-2% PFA fixed, permeabilized tissues or cell cultures. Mild pepsin digestion improves staining.
Immunohistochemistry (formalin-fixed and paraffin-embedded sections): 1:200-1:400.
Requires pepsin digestion (0.1-.4% pepsin in .1% HCL pH 1.0, 30-50 minutes at 37C; neutralize sections with 2 quick rinses in 1X PBS pH 7.4 prior to blocking and antibody staining. Enhanced ABC or amplified detection systems recommended for best signals. See Soini, Y 1993 J Clin Patholo for protocol for decalified bone.
Immunoblotting: 1:1000; SDS sample buffer lysis of whole human fibroblasts; 6.5% PAGE reduced gels. Two distinct bands, 250,000 & 190,000 {Soini, Y Am. J. Clin. Path. 1993)
Final dilutions must be determined by end user.
Immunohistochemistry (formalin-fixed and paraffin-embedded sections): 1:200-1:400.
Requires pepsin digestion (0.1-.4% pepsin in .1% HCL pH 1.0, 30-50 minutes at 37C; neutralize sections with 2 quick rinses in 1X PBS pH 7.4 prior to blocking and antibody staining. Enhanced ABC or amplified detection systems recommended for best signals. See Soini, Y 1993 J Clin Patholo for protocol for decalified bone.
Immunoblotting: 1:1000; SDS sample buffer lysis of whole human fibroblasts; 6.5% PAGE reduced gels. Two distinct bands, 250,000 & 190,000 {Soini, Y Am. J. Clin. Path. 1993)
Final dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Linkage
Replaces: MAB1902
Physical form
Format: Purified
The purified antibody is in PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.
Storage and Stability
Maintain refrigerated at 2-8°C
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Tenascin immunoreactivity as a prognostic marker in usual interstitial pneumonia.
Kaarteenaho-Wiik, R, et al.
American Journal of Respiratory and Critical Care Medicine, 154, 511-518 (1996)
Tenascin expression in mucocutaneous diseases and related lesions of human oral mucosa.
Tiitta, O, et al.
Archives of Oral Biology, 40, 1039-1045 (1995)
Y Soini et al.
Journal of clinical pathology, 46(3), 218-221 (1993-03-01)
To determine the distribution of tenascin in normal and pathological bone marrow. 48 different bone marrow lesions were studied immunohistochemically using a monoclonal antibody to tenascin. Tenascin immunoreactivity was found in lesions with increased fibrosis and high numbers of reticular
Demonstration of tenascin-like immunoreactivity in rabbit corneal wounds.
Tervo, K, et al.
Acta Ophthalmologica, 67, 347-350 (1989)
Pleural mesotheliomas have an integrin profile distinct from visceral carcinomas.
Koukoulis, G K, et al.
Human Pathology, 28, 84-90 (1997)
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