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Key Documents

MAB1687

Sigma-Aldrich

Anti-Vimentin Antibody, clone 43BE8

ascites fluid, clone 43BE8, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

43BE8, monoclonal

species reactivity

human, monkey

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)

isotype

IgM

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... VIM(7431)

Specificity

Vimentin (58kD). Reacts in immunocytochemical assays with all mesenchymal cells (fibroblasts, Scwann cells, endothelial cells, smooth muscle cells, etc.).

Application

Immunohistochemistry: 1:500-1:2,000 on paraffin embedded sections (ABC peroxidase) fixed with alcohol (e.g. Carnoy) with a 2" pronase digestion. Aldehyde fixation is not recommended.

Immunocytochemistry: 1:100-1:200 cells in vitro (IFA) fixed in ice cold absolute methanol or acetone.

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Vimentin Antibody, clone 43BE8 is validated for use in IC, IH(P) for the detection of Vimentin.

Physical form

Liquid

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Spontaneous transformation of adult mesenchymal stem cells from cynomolgus macaques in vitro.
Ren Z, Wang J, Zhu W, Guan Y, Zou C, Chen Z, Zhang YA
Experimental Cell Research null
Anja Torsvik et al.
Experimental cell research, 318(5), 441-443 (2012-01-10)
There is at present a controversy in the literature whether MSCs are susceptible to spontaneous in vitro transformation or not. Several groups have reported spontaneous transformation of MSCs from various species. However, some of these reports were not true transformations
Skin Rejuvenation with Cultured Melanocyte and Fibroblasts in a Medical Tourism Patient.
Atefeh Shahbazi et al.
World journal of plastic surgery, 11(3), 116-121 (2023-01-26)

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