Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

IF014

Sigma-Aldrich

Interferon-β Protein, Recombinant human

Recombinant Human Interferon Beta 1a (Hu-IFNbeta 1a).

Synonym(s):

Fibroblast Interferon

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in CHO cells

assay

≥95% (one N-glycosylation site)

form

liquid

specific activity

3.99 x 108 U/mg

manufacturer/tradename

Chemicon®

concentration

1 x 106 units/mL

technique(s)

cell culture | mammalian: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

General description

Product Source: cDNA obtained from human fibroblast mRNA expressed in CHO cells.
Recombinant Human Interferon Beta 1a (Hu-IFNbeta 1a). Molecular weight is ~23 kDa.

Linkage

Replaces: IF008

Physical form

Frozen liquid in 50 mM NaOAc, pH 5.5, containing 0.1% BSA.

Storage and Stability

After receipt, this product should be kept at -70°C or below for retention of full activity.
Thaw product vial by incubation cold tap water until just thawed. Thawed IFNβ should be
stored in working aliquots at -70°C for up to six months. Avoid multiple freeze/thaw cycles.
Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the
product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Analysis Note

Specific Activity: Determined by assaying inhibition of virus-induced cytopathic effect.In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%.The units are determined with respect to the international reference standard for human interferon beta (Hu-IFNbeta) provided by the National Institutes of Health.Unit of activity measured on VERO cells with vesicular stomatitis virus (VSV).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Colton R Martens et al.
PLoS pathogens, 18(2), e1010342-e1010342 (2022-02-23)
Viral infection of the heart is a common but underappreciated cause of heart failure. Viruses can cause direct cardiac damage by lysing infected cardiomyocytes. Inflammatory immune responses that limit viral replication can also indirectly cause damage during infection, making regulatory
Zhaowei Chu et al.
Nature communications, 13(1), 1859-1859 (2022-04-08)
The cohesin complex participates in the organization of 3D genome through generating and maintaining DNA loops. Stromal antigen 2 (STAG2), a core subunit of the cohesin complex, is frequently mutated in various cancers. However, the impact of STAG2 inactivation on
David A Constant et al.
ImmunoHorizons, 6(7), 416-429 (2022-07-06)
The three types of IFN have roles in antimicrobial immunity and inflammation that must be properly balanced to maintain tissue homeostasis. For example, IFNs are elevated in the context of inflammatory bowel disease and may synergize with inflammatory cytokines such
Maximilian Hirschenberger et al.
STAR protocols, 2(4), 100781-100781 (2021-08-19)
We present a protocol for analyzing the impact of SARS-CoV-2 proteins in interferon signaling using luciferase reporter assays. Here, the induction of defined promoters can be quantitatively assessed with high sensitivity and broad linear range. The results are similar to
Manuel Hayn et al.
Cell reports, 35(7), 109126-109126 (2021-05-12)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) evades most innate immune responses but may still be vulnerable to some. Here, we systematically analyze the impact of SARS-CoV-2 proteins on interferon (IFN) responses and autophagy. We show that SARS-CoV-2 proteins synergize

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service