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MILLIPLEX® Human Soluble Cytokine Receptor Panel- Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

Synonym(s):

Human Cytokine Receptor Multiplex Kit, Luminex® Human Cytokine Receptor Immunoassay, Millipore Cytokine Receptor Panel

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 91-104%
sensitivity: 4-208 pg/mL
(Overnight Incubation minDC)
standard curve range: 12.2-50,000 pg/mL
(sIL-4R, sIL-6R, sRAGE, sTNFRI, sTNFRII)
standard curve range: 122.1-500,000 pg/mL
(sEGFR, sIL-1RII, sVEGF-R1, sVEGF-R2, sVEGF-R3)
standard curve range: 24.4-100,000 pg/mL
(sCD30, sgp130, sIL-1RI, sIL-2Rα)
inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

Cytokine receptors constitute an integral part of cytokine biology. Like cytokines, cytokine receptors are involved in normal physiological and pathological processes of almost all disease states. Soluble cytokine receptors naturally arise from genes encoding membrane- bound receptors or are direct derivatives of the receptors themselves. The discovery that soluble cytokine receptors are involved in regulating excessive inflammatory responses and modulating immune events has stimulated significant research interest in their potential role as immunotherapeutic agents. Many of these soluble cytokine receptors can inhibit the binding and biological activity of their cytokine ligands, making them very specific cytokine antagonists.

MILLIPLEX® Human Soluble Cytokine Receptor Panel is to be used for the simultaneous quantification of 14 soluble cytokine receptors in human serum, plasma and cell / tissue culture supernatant samples. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Application

  • Analytes: sCD30, sEGFR, sGP130, sIL-1RI, sIL-1RII, sIL-2Rα, sIL-4R, sIL-6R, sRAGE, sTNFRI, sTNFRII, sVEGFR1, sVEGFR2, sVEGFR3
  • Recommended Sample type: Serum, plasma or tissue/cell lysate and culture supernatant
  • Recommended Sample dilution: 1:5 dilution for plasma or serum or neat cell culture supernatant
  • Assay Run Time: Overnight
  • Research Category: Inflammation & Immunology

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.
Sensitivity: Please see kit protocol for individual assay sensitivities.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

Certificates of Analysis (COA)

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Bryan D Young et al.
International heart journal, 62(5), 1096-1105 (2021-09-22)
While cardiac imaging has improved the diagnosis and risk assessment for cardiac sarcoidosis (CS), treatment regimens have consisted of generalized heart failure therapies and non-specific anti-inflammatory regimens. The overall goal of this study was to perform high-sensitivity plasma profiling of
Sandra Hellberg et al.
Cell reports, 16(11), 2928-2939 (2016-09-15)
Multiple sclerosis (MS) is a chronic inflammatory disease of the CNS and has a varying disease course as well as variable response to treatment. Biomarkers may therefore aid personalized treatment. We tested whether in vitro activation of MS patient-derived CD4+ T cells could
Dai Kimura et al.
Frontiers in pediatrics, 4, 31-31 (2016-04-12)
Lung injury activates multiple pro-inflammatory pathways, including neutrophils, epithelial, and endothelial injury, and coagulation factors leading to acute respiratory distress syndrome (ARDS). Low-dose methylprednisolone therapy (MPT) improved oxygenation and ventilation in early pediatric ARDS without altering duration of mechanical ventilation
Adrián Montes-de-Oca-García et al.
Frontiers in physiology, 13, 885185-885185 (2022-08-09)
This study aimed to analyze the influence of the peroxisome proliferator-activated receptor (PPAR)-gamma coactivator (PGC)-1 alpha (PPARGC1A) gene rs8192678 C>T polymorphism on different health-related parameters in male and female young adults. The PPARGC1A gene rs8192678 polymorphism was ascertained by polymerase

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service