AP113A
Goat Anti-Human IgG Antibody, Fc, Alkaline Phosphatase conjugate
Chemicon®, from goat
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Recommended Products
biological source
goat
Quality Level
conjugate
alkaline phosphatase conjugate
antibody form
affinity purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
Related Categories
General description
Alkaline Phosphatase-conjugated Affinity Purified Goat anti-Human IgG
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Specificity
Based on immunoelectrophoresis, the antibody reacts with the heavy chain of human IgG but not with the light chains of most human immunoglobulins.. No antibody was detected against normal human IgM or IgA, or against non-immunoglobulin serum proteins, but the antibody may cross-react with immunoglobulins from other species.
Application
Goat anti-Human IgG Antibody, Fc, Alkaline Phosphatase conjugate detects level of Human IgG & has been published & validated for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Fragment Specific Secondary Antibodies
Western Blot:
1:5,000-1:50,000 dilution can be used.
Optimal working dilutions must be determined by the end user.
1:5,000-1:50,000 dilution can be used.
Optimal working dilutions must be determined by the end user.
Quality
Routinely evaluated by Enzyme-linked Immunosorbent Assay.
ELISA:
1:5,000-1:50,000 dilution can be used.
ELISA:
1:5,000-1:50,000 dilution can be used.
Linkage
Replaces: MABN1055
Physical form
Goat secondary antibody IgG in Lyophilized Buffer containing 0.01 M Tris HCl, 0.25 M NaCl, pH 8.0 with 15 mg/mL BSA and 0.05% sodium azide.
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.
Unpurified
Storage and Stability
Stable for 1 year at 2–8°C from date of receipt.
After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade for better) to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade for better) to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
hcodes
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
Certificates of Analysis (COA)
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Jepson, S; Vought, B; Gross, CH; Gan, L; Austen, D; Frantz, JD; Zwahlen, J; Lowe et al.
The Journal of Biological Chemistry null
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