ABT95

Anti-TGN46, Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Trans-Golgi network integral membrane protein 2, TGN38 homolog, TGN46, TGN48, Trans-Golgi network protein TGN51
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, rat, human
• species reactivity (predicted by homology): canine (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_006455
• UniProt accession no.: O43493
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TGOLN2(10618)

Description

General description

TGN46 is one of several proteins that utilize retrograde transport to travel between the trans-Golgi network (TGN) and the endocytic pathway. Retrograde transport involves the tethering, docking, fusion, and post translational modification of TGN proteins. Further studies are still required to elucidate the roles and mode of action for TGN46.

Specificity

This antibody recognizes the cytoplasmic domain of TGN46.

Immunogen

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human TGN46.

Application

Anti-TGN46 detects levels of TGN46 proteins & has been published & validated for use in wb, IP & ICC.

Research Category
Cell Structure

Research Sub Category
Vesicular Trafficking

Western Blot Analysis: 2 µg/mL of this antibody detected TGN46 in 10 µg of NIH/3T3 cell lysate. Multiple uncharacterized bands may be observed in some cell lysates.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected TGN46 in A431 and HeLa cells.

Quality

Evaluated by Immunoprecipitation in HeLa cell lysate.

Immunoprecipitation Analysis: 0.2 µg of this antibody immunoprecipitated TGN46 in 500 µg of HeLa cell lysate (An uncharacterized band at ~29 kDa may be observed in some cell lysates.).

Target description

~110 kDa observed. This protein is observed at ~110 kDa, and it is subject to glycosylation (Khanobdee, K. et al. (2004). Mol Vis. 13(10):933-42.; Prescott, A. R., et al. (1997). Eur J Cell Biol. 72(3):238-246.).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable