ABS1652

Anti-GSTA4 Antibody

serum, from rabbit

• Synonym(s): Glutathione S-transferase alpha-4, Glutathione S-transferase A4, Glutathione S-transferase Yk, GSTA4, GST 8-8, GST A4-4, GST K, GST Yk
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, rat
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI accession no.: NP_001100310
• UniProt accession no.: P14942
• shipped in: dry ice
• target post-translational modification: unmodified

Description

General description

Glutathione S-transferase alpha-4 (EC:2.5.1.18; UniProt P14942; also known as Glutathione S-transferase A4, Glutathione S-transferase Yk, GST 8-8, GST A4-4, GST K, GST Yk) is encoded by the Gsta4 gene (Gene ID 300850) in rat. Glutathione S-transferases constitute a family of isozymes that are involved in detoxification by catalyzing the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates. The GST family can be subdivided into the cytosolic, mitochondrial, and microsomal (a.k.a. MAPEG) proteins. Mammalian GSTs exist either as homodimers or heterodimers. The individual monomers were historically characterized by their quaternary structure and size, Yf (24.5 kDa), Yk (25 kDa), Ya1 (25.5 kDa), Ya2 (25.5 kDa), Yn (26.5 kDa), Yb1 (27 kDa), Yb2 (27 kDa) and Yc (28.5 kDa). The Y designation refers to the Y fraction (as opposed to the X and Z fractions) that were found during purification by Sephadex G75 chromatography. Two GST superfamilies exist that comprise either cytosolic or membrane-bound proteins. Evidence suggests that cytosolic GST increases bodily resistance to carcinogens, products of oxidative stress, environmental pollutants and certain anti-cancer drugs. The cytosolic GSTs are further subdivided into eight classes, namely alpha (GSTA), mu (GSTM), pi (GSTP), sigma (GSTS), theta (GSTT), kappa (GSTK), zeta (GSTZ), and omega (GSTO). GSTA4 exists as a homodimer composed of two Yk subunits.

Specificity

This antiserum selectively reacts with Yk from purified rat liver GST K and rat kidney GST J. No reactivity toward Ya, Yc, Yb1, Yb2, Yb3, Yf, or Yn subunits from other forms of purified rat GSTs (Hayes, J.D,, and Mantle, T.J. (1986). Biochem. J. 233(3):779-788).

Immunogen

Purified rat liver GST K (YkYk).

Application

Anti-GSTA4 Antibody is an antibody against GSTA4 for use in Western Blotting, Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GSTA4 in rat kidney, rat liver, and mouse cerebral cortex tissue.
Western Blotting Analysis: A representative lot detected upregulated Glutathione S-transferase alpha-4 in liver cytosolic preparations from mice on high-fat diet (F) when compared with liver samples from mice on regular diet (Meakin, P.J., et al. (2014). Mol. Cell. Biol. 34(17):3305-3320).
Western Blotting Analysis: A representative lot detected upregulated Glutathione S-transferase alpha-4 levels in liver homogenates from mice fed on diets supplemented with the antioxidant butylated hydroxyanisol (BHA) (Chanas, S.A., et al. (2002). Biochem. J. 365:405-416).
Western Blotting Analysis: A representative lot detected purified rat liver GST K (YkYk) and kidney GST J (YkYk), but not Ya, Yc, Yb1, Yb2, Yb3, Yf, or Yn subunits from other forms of purified rat GSTs (Hayes, J.D,, and Mantle, T.J. (1986). Biochem. J. 233(3):779-788).

Research Category
Signaling

Research Sub Category
Oxidative Stress

Quality

Evaluated by Western Blotting in rat kidney tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected GSTA4 in 10 µg of rat kidney tissue lysate.

Target description

~25 kDa observed.

Physical form

Rabbit polyclonal antibody in serum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable