ABE2984

Anti-Phospho-RXRα/RXRA (Ser56)

• Synonym(s): Nuclear receptor subfamily 2 group B member 1, Retinoic acid receptor RXR-alpha, Retinoid X receptor alpha
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• mol wt: calculated mol wt 50.81 kDa; observed mol wt ~75-80 kDa
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): bovine, rat, mouse
• packaging: antibody small pack of 100
• technique(s): dot blot: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal
• Protein ID accession no.: NP_001278850.1
• UniProt accession no.: P19793
• storage temp.: 2-8°C
• Gene Information: human ... RXRA(6256)

Description

Specificity

This rabbit polyclonal antibody detects RXR phosphorylated on serine 56.

Immunogen

KLH-conjugated linear peptide corresponding to 12 amino acids from the surrounding phosphoserine 56 from the N-terminal region of human Retinoic acid receptor (RXR ).

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from HeLa cells in mitotic phase.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RXR phosphorylated on serine 56 in lysate from HeLa cells in mitotic phase.

Tested Applications

Immunofluorescence Analysis: A representative lot detected RXR in Immunofluorescence application (Xie, G., et al. (2021). STAR Protocols. 2, :1-15).

Western Blotting Analysis: A representative lot detected RXR in Western Blotting application (Xie, G., et al. (2020). Development Cell. 55, : 707-722).

Immunoprecipitation Analysis: A representative lot immunoprecipitated RXR in Immunoprecipitation application (Xie, G., et al. (2020). Development Cell. 55, : 707-722).

Immunocytochemistry Analysis: A representative lot detected RXR in Immunocytochemistry application (Xie, G., et al. (2020). Development Cell. 55, : 707-722).

Flow Cytometry Analysis: A representative lot detected RXR in Flow Cytometry application (Xie, G., et al. (2020). Development Cell. 55, : 707-722).

Dot Blot: A representative lot detected RXR in Dot Blot application (Xie, G., et al. (2020). Development Cell. 55, : 707-722).

Flow Cytometry Analysis: 1.0 µg of this antibody detected RXR in HeLa cells overnight serum-starved and treated with Nocodazole 100 ng/ml for 8 hours .

Immunohistochemistry Applications: A representative lot detected RXR in Immunohistochemistry application (Xie, G., et al. (2020). Development Cell. 55, : 707-722).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Retinoic acid receptor RXR-alpha (UniProt: P19793; also known as Nuclear receptor subfamily 2 group B member 1, Retinoid X receptor alpha, RXR ) is encoded by the RXRA (also known as NR2B1) gene (Gene ID: 6256) in human. Retinoid X receptor alpha (RXR ), a nuclear receptor of transcription factor, controls various physiological and pathological pathways including cellular growth, proliferation, differentiation, and apoptosis. RXR-alpha is composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain, and a C-terminal ligand-binding domain (AF2 domain). RXR-alpha protein is highly expressed in liver and its expression has also been reported in lung fibroblasts, monocytes, kidney, and brain. RXR is phosphorylated on serine and threonine residues mainly at its N-terminal A/B domain by cyclin-dependent kinase 1 (Cdk1) at the onset of mitosis, triggering its translocation to the centrosome, where phosphorylated-RXR (p-RXR ) interacts with polo-like kinase 1 (PLK1) through its N-terminal A/B domain by a unique mechanism. The interaction promotes PLK1 activation, centrosome maturation, and mitotic progression. Studies show that Cdk1 phosphorylation of Ser56 and Ser70 mediates the interaction between p-RXR and PLK1 during mitosis. Importantly, Ser56 and Ser70 and their surrounding amino acids are highly conserved across different species, suggesting their evolutionarily conserved role in regulating RXR activity. An RXR ligand XS060, which specifically inhibits p-RXR /PLK1 interaction but not RXR heterodimerization, promotes mitotic arrest and catastrophe in a tumor-specific manner. Levels of p-RXR are abnormally elevated in cancer cell lines, during carcinogenesis in animals, and in clinical tumor tissues. (Ref.: Xie, G., et al. (2020). Development Cell. 55(6); 707-722; Xie, G., et al. (2021). STAR Protocols. 2(3); 1-15; Zeng, Z., et al. (2015). Cancer Res. 75(10); 2049-2060.)

Physical form

Purified rabbit polyclonal antibody in PBS without azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable