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Key Documents

ABC67

Sigma-Aldrich

Anti-Mitochondrial fission 1 protein (Fis1) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

FIS1 homolog, hFis1, Tetratricopeptide repeat protein 11, TPR repeat protein 11

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

western blot: suitable

NCBI accession no.

NP_057152

UniProt accession no.

Q9Y3D6

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FIS1(51024)

General description

Mitochondrial fission 1 protein, hFIs1, is an overexpressed mammlian protein that is localized to the outer mitochondrial membrane of various cell types. It is involved in the fragmentation of the mitochondrial network and its perinuclear clustering, resulting in the release of cytochrome c from the mitochondrion, and apopotosis. It is known to mediate peroxisomal fission and also plays a critical role in histone function by demethylating Lys-4 of histone H3.

Specificity

Other homologies: Rat and Mouse (83% sequence homology).
This antibody recognizes the cytoplasmic topological domain of Mitochondrial fission 1 protein.

Immunogen

Epitope: Cytoplasmic topological domain
KLH-conjugated-linear peptide corresponding to the cytoplasmic topological domain of human Mitochondrial fission 1 protein.

Application

Detect Mitochondrial fission 1 protein (Fis1) using this Anti-Mitochondrial fission 1 protein (Fis1) Antibody validated for use in WB.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blot in Raji cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Mitochondrial fission 1 protein in 10 µg of Raji cell lysate.

Target description

~17 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Raji cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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