ABC458

Anti-Thymidylate Kinase/TMPK Antibody

from rabbit

• Synonym(s): Thymidylate kinase, dTMP kinase
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_036277
• UniProt accession no.: P23919
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TK1(7083)

Description

General description

TMPK, also known as dTMP kinase, and encoded by the gene DTYMK/CDC8/TMPK/TYMK, is a key enzyme for pyrimidine synthesis that catalyzes the phosphorylation of thymidine 5′-monophosphate (dTMP) to form thymidine 5′-diphosphate (dTDP). TMPK has important functions in cell proliferation and DNA replication, and the synthesis of dTDP is unique because it requires TMPK. All other dNDPs are directly produced by ribonucleotide reductase (RNR). Thus, TMPK is well recognized as a potential drug target for many diseases and disease agents including viruses. TMPK is currently a target of anti-HIV nucleoside therapy, and TMPK is also a validated target for antibiotic development against resistance strains of bacteria and parasites, and research demonstrates that it may also be a good target for the anticancer agent doxorubicin cancer cells. Expressed by virtually all cells it is localized to cell cytoplasm with only traces found in Golgi, mitochondria and nuclei.

Immunogen

GST-tagged recombinant protein corresponding to human Thymidylate Kinase/TMPK.

Application

Anti-Thymidylate Kinase/TMPK Antibody is a rabbit polyclonal antibody, validated for use in western blotting & IP.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: A representative lot from an independent laboratory detected Thymidylate Kinase/TMPK in MDA-MB231, MCF-7, H184B5F5/M10 cell lysates (Hu, C. M., et al. (2012). Cancer Cell. 22(1):36-50.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunprecipitated Thymidylate Kinase/TMPK from MCF-7, H184B4F5/M10, and MCF-10A cell lysates (Ke, P. Y., et al. (2005). Genes Dev. 19(16):1920-1933.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Thymidylate Kinase/TMPK in MCF-7, H184B4F5/M10, and MCF-10A cell lysates (Ke, P. Y., et al. (2005). Genes Dev. 19(16):1920-1933.).

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Thymidylate Kinase/TMPK in 10 µg of Jurkat cell lysate.

Target description

~24 kDa observed

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable