AB7824
Anti-p73 Antibody, α and β
Chemicon®, from rabbit
Synonym(s):
p53-like Transcription Factor
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HSPA8(3312)
Specificity
Antibody AB7824 recognizes amino acids 450-636 of p73 protein. This region is common to both p73alpha and p73beta isoforms, hence both isoforms of the p73 protein will be detected.
Immunogen
Epitope: alpha and beta
GST fusion protein containing aa450-636 of p73 protein.
Application
Anti-p73 Antibody, α & β is a Rabbit Polyclonal Antibody for detection of p73 also known as p53-like Transcription Factor & has been validated in IP, WB, IHC(P).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Western blot: 1 μg/mL of the antibody will detect p73 in 30μg of total cell lysate from overexpressing cell lines. Higher levels of the antibody may be necessary when using native tissue sources.
Immunoprecipitation: Add 1μg of antibody to a sample that has been precleared with Protein A Sepharose™ in 500 μl lysis buffer. Incubate antibody with sample at 4°C for 2 hours with agitation. Follow by incubation with 50 μL Protein A Sepharose for 2 hours at 4°C with agitation. Collect the Protein A complex by centrifugation, washing pellet 3 times in lysis buffer. Resuspend final sample in electrophesis buffer, denature by heating at 95°C for 5 minutes, and analyze by SDS-PAGE
Immunohistochemistry of paraffin sections: Signal amplification may be necessary, in conjunction with standard IHC procedures. Dewax and rehydrate sections through descending graded alcohols to water using standard dewaxing protocols. Subject section to heat antigen retrieval. Wash sections 3 times with TBS, pH 7.4. Block endogenous peroxidase activity by incubating the section in 1% H2O2 in methanol for 30 minutes. Wash with TBS and detect according to preferred detection methods. With high sensitivity detection systems, typical working dilutions for the p73 antibody are 1:5,000 - 1;10,000.
Optimal working dilutions must be determined by end user.
Immunoprecipitation: Add 1μg of antibody to a sample that has been precleared with Protein A Sepharose™ in 500 μl lysis buffer. Incubate antibody with sample at 4°C for 2 hours with agitation. Follow by incubation with 50 μL Protein A Sepharose for 2 hours at 4°C with agitation. Collect the Protein A complex by centrifugation, washing pellet 3 times in lysis buffer. Resuspend final sample in electrophesis buffer, denature by heating at 95°C for 5 minutes, and analyze by SDS-PAGE
Immunohistochemistry of paraffin sections: Signal amplification may be necessary, in conjunction with standard IHC procedures. Dewax and rehydrate sections through descending graded alcohols to water using standard dewaxing protocols. Subject section to heat antigen retrieval. Wash sections 3 times with TBS, pH 7.4. Block endogenous peroxidase activity by incubating the section in 1% H2O2 in methanol for 30 minutes. Wash with TBS and detect according to preferred detection methods. With high sensitivity detection systems, typical working dilutions for the p73 antibody are 1:5,000 - 1;10,000.
Optimal working dilutions must be determined by end user.
Physical form
Format: Purified
Purified immunoglobulin. 0.22 micron filtered. Provided at 1 mg/mL in PBS, pH 7.4, containing 0.05% sodium azide.
Storage and Stability
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Expression of p73 in normal skin and proliferative skin lesions.
Makoto Kamiya, Yuko Takeuchi, Mari Katho, Hideaki Yokoo, Atsushi Sasaki, Yoichi Nakazato
Pathology International null
Differential expression of two new members of the p53 family, p63 and p73, in extramammary Paget's disease.
S Chen, Y Moroi, K Urabe, S Takeuchi, M Kido, S Hayashida, H Uchi, T Uenotsuchi, Y Tu, M Furue
Clinical and Experimental Dermatology null
The expression of p73, p21 and MDM2 proteins in gliomas.
Makoto Kamiya, Yoichi Nakazato, Makoto Kamiya, Yoichi Nakazato
Journal of Neuro-Oncology null
Differential expression of p53 gene family members p63 and p73 in head and neck squamous tumorigenesis.
Hong-Ran Choi, John G Batsakis, Feng Zhan, Erich Sturgis, Mario A Luna, Adel K El-Naggar
Human Pathology null
Overexpression of phosphorylated-ATF2 and STAT3 in cutaneous angiosarcoma and pyogenic granuloma.
Si-Yuan Chen,Satoshi Takeuchi,Kazunori Urabe,Sayaka Hayashida,Makiko Kido,Hiroto Tomoeda et al.
Journal of cutaneous pathology null
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service