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Key Documents

AB1559

Sigma-Aldrich

Anti-Adenosine A2a Receptor Antibody

serum, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

pig, bovine, rat, human, canine

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

suitability

not suitable for immunohistochemistry

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ADORA2A(135)

Immunogen

Synthetic 30 amino acid peptide corresponding to amino acids 383-412 of the canine adenosine A2a receptor near the C-terminal intracellular domain (30 amino acids) of the cloned canine A2a receptor. The A2a sequence selected in not present in A1, A2b and A3 receptors.

Application

This Anti-Adenosine A2a Receptor Antibody is validated for use in ELISA, WB for the detection of Adenosine A2a Receptor.
Western blot: 1:1,000-1:5,000 (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.

ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG285)/well)

Optimal working dilutions must be determined by end user.

Physical form

Rabbit antiserum. Liquid containing 0.1% sodium azide.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Astrocytic adenosine A2A receptors control the amyloid-? peptide-induced decrease of glutamate uptake.
Matos, Marco, et al.
Journal of Alzheimer'S Disease, 31, 555-567 (2012)
M E Olah et al.
Annual review of pharmacology and toxicology, 35, 581-606 (1995-01-01)
Adenosine receptors (ARs) are members of the G protein-coupled receptor family and mediate the multiple physiological effects of adenosine. Currently, four AR subtypes have been cloned: A1AR, A2aAR, A2bAR, and A3AR. All subtypes are distinctly distributed throughout the body and
Neurochemical and structural organization of the principal nucleus of the inferior olive in the human.
Joan S Baizer,Chet C Sherwood,Patrick R Hof,Sandra F Witelson,Fahad Sultan
Anatomical Record (Hoboken, N.J. : 2007) (2007)
Rod Phosphodiesterase-6 ( PDE6) Catalytic Subunits Restore Cone Function in a Mouse Model Lacking Cone PDE6 Catalytic sty
Kolandaivelu S, Chang B, Ramamurthy V
The Journal of Biological Chemistry null
Differential expression of adenosine receptors in human neutrophils: up-regulation by specific Th1 cytokines and lipopolysaccharide.
Fortin, A; Harbour, D; Fernandes, M; Borgeat, P; Bourgoin, S
Journal of Leukocyte Biology null

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