70979
BetaFluor β-Galactosidase Assay Kit
Optimized reagents for ultrasensitive β-gal assays
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About This Item
UNSPSC Code:
41106513
NACRES:
NA.54
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General description
Optimized reagents for ultrasensitive β-gal assays
The BetaFluor β-Gal Assay Kit is designed for rapid, sensitive measurement of β-galactosidase activity in cell extracts. The kit features Reportasol Extraction Buffer, which enables efficient passive extraction of cell protein while maintaining optimal enzyme activity, and is configured for 96-well format. Because no mechanical operations are necessary for extraction, the assay is highly amenable to high-throughput reporter gene expression analysis. The BetaFluor Assay is designed for fluorometric detection with 500-fg sensitivity.
Components
•100 mgBetaFluor Substrate
•25 mlReportasol Extraction Buffer
•100 mlReaction Buffer
•50 mlStop Buffer
•1.6 ml1 M DTT
•25 mlReportasol Extraction Buffer
•100 mlReaction Buffer
•50 mlStop Buffer
•1.6 ml1 M DTT
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
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Danger
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Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
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Justine M Pinskey et al.
The Journal of biological chemistry, 292(37), 15192-15204 (2017-07-02)
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