681660-M

N-WASP-Inhibitor, 187-1

The N-WASP-Inhibitor, 187-1 controls the biological activity of neural Wiskott Aldrich syndrome protein (N-WASP). This small molecule/inhibitor is primarily used for Cell Structure applications.

• Synonym(s): N-WASP-Inhibitor, 187-1, cyclo( LK DF DP DF LF DP LQ)₂
• Empirical Formula (Hill Notation): C₉₆H₁₂₂N₁₈O₁₆
• Molecular Weight: 1784.11
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• color: white
• solubility: DMSO: 1 mg/mL; acetic acid: 5%
• storage temp.: −20°C

Description

General description

A 14-amino acid cyclic peptide that potently blocks phosphatidylinositol 4,5-bisphosphate (PIP₂)-induced actin assembly in Xenopus egg cytoplasmic extract (IC₅₀ ~ 2 µM). Shown to directly bind to neural Wiskott Aldrich syndrome protein (N-WASP) with high-affinity, and prevent the activation of actin-related protein 2/3 (Arp2/3) complex by inhibiting the interaction between N-WASP and Arp2/3.

A 14-amino acid cyclic peptide that potently blocks phosphatidylinositol 4,5-bisphosphate (PIP₂)-induced actin assembly in Xenopus egg cytoplasmic extract (IC₅₀ ~2 µM). Shown to directly bind to neural Wiskott Aldrich syndrome protein (N-WASP) with high-affinity, and allosterically stabilize the autoinhibited conformation of N-WASP. It prevents the activation of actin-related protein 2/3 (Arp2/3) complex by blocking the interaction between N-WASP and Arp2/3.

Biochem/physiol Actions

Primary Target
Neural Wiskott Aldrich syndrome protein (N-WASP)

Target IC₅₀: ~ 2 µM blocking phosphatidylinositol 4,5-bisphosphate (PIP2)-induced actin assembly in Xenopus egg cytoplasmic extract

Warning

Toxicity: Standard Handling (A)

Sequence

cyclo(LLys-DPhe-DPro-DPhe-LPhe-DPro-LGln)₂

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Other Notes

Peterson, J.R., et al. 2001. Proc. Natl. Acad. Sci. USA98, 10624.
Suetsugu, S., et al. 2001. J. Biol. Chem.276, 33175.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable