565758

β-Secretase Substrate IV, Fluorogenic

≥95% (HPLC), lyophilized solid, β-secretase substrate, Calbiochem^®

• Synonym(s): β-Secretase Substrate IV, Fluorogenic, FS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic
• Empirical Formula (Hill Notation): C₉₁H₁₂₉N₂₅O₂₅S
• CAS Number: 310427-94-2
• Molecular Weight: 2005.22
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• product name: β-Secretase Substrate IV, Fluorogenic, A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP).
• Quality Level: 100
• assay: ≥95% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: red
• solubility: DMSO: 5 mg/mL
• fluorescence: λ_ex ~350 nm; λ_em ~490 nm
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm; emission max.: ~490), based on the GVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by β-secretase results in fluorescence enhancement. Reported K_cat/k_m values (min^-1/µM) for pro-mempasin 2 and its proteolytically activated species Leu^28p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37°C).

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement. k_cat/K_m values (min^-1/µM) at 37°C, pH 4.5, for pro-memapsin 2 and its activated species Leu^28p-memapsin 2 are 0.044 and 0.040, respectively.

Biochem/physiol Actions

Cell permeable: no

Primary Target
β-secretase substrate

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.

Other Notes

Ermolieff, J., et al. 2000. Biochemistry 39, 12450.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable