5.00554

mTOR Activator, MHY1485

• Synonym(s): mTOR Activator, MHY1485, 4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine, Autophagy Inhibitor III
• Empirical Formula (Hill Notation): C₁₇H₂₁N₇O₄
• CAS Number: 326914-06-1
• Molecular Weight: 387.39
• UNSPSC Code: 12352200
• PubChem Substance ID: 2026662

Properties

• assay: ≥97% (HPLC)
• Quality Level: 100
• form: solid
• potency: ~2 μM IC₅₀
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: pale yellow
• solubility: DMSO: 12.5 mg/mL
• storage temp.: 2-8°C
• SMILES string: C1CCN(CC1)S(=O)(=O)C2=CC=C(C=C2)NC(=O)C3=CC(=NN3)C4=CC=CC=C4O

Description

General description

A cell-permeable triazine derivative that is shown to increase cellular phosphorylation level of mTOR Ser2448 (20% and 42% above basal in 1 h, respectively, with 1 and 2 µM MHY1485) and downstream substrate 4E-BP Thr37/46 (29% and 34% above basal in 1 h, respectively, with 1 and 2 µM MHY1485) in rat liver Ac2F cells, affecting culture viability only at much higher concentrations (by 20% after 24 h 20 µM treatment). MHY1485 induced cellular LC3-II accumulation in Ac2F (117%, 150%, and 250% of vehicle control LC3-II/LC3-I ratio, respectively, with 0.5, 1, and 2 µM treatment for 6 h; 142% and 172% of control ratio, respectively, with 2 µM treatment for 1 h or 12 h) is reported to be a result of autophagy inhibition due to reduced fusion between autophagosomes and lysosomes.

Biochem/physiol Actions

Cell permeable: yes

Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Choi, Y. J., et al. 2012. PLoS. One.7, e43418.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable