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401902

Sigma-Aldrich

Goat Anti-Human IgM, μ-Chain Specific Alkaline Phosphatase Conjugate

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.77

biological source

goat

Quality Level

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

General description

Immunoaffinity purified goat polyclonal antibody conjugated to alkaline phosphatase. Recognizes human IgM μ-chain.
This Goat Anti-Human IgM, µ-Chain Specific Alkaline Phosphatase Conjugate is validated for use in Enzyme Immunoassay, Immunoelectrophoresis for the detection of Human IgM, µ-Chain Specific.

Immunogen

Human

Application

Enzyme Immunoassay (1:5000)

Immunoelectrophoresis (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM Tris-HCl, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, pH 7.6.

Other Notes

Monospecific for human IgM μ-chain as determined by immunoelectrophoresis against normal human serum. p-nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C was used as substrate for testing immunoenzyme reactivity. Antibody is applicable in any immunoassay requiring an alkaline phosphatase conjugated secondary antibody. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Malick M Gibani et al.
Nature medicine, 25(7), 1082-1088 (2019-07-05)
Salmonella Typhi is a human host-restricted pathogen that is responsible for typhoid fever in approximately 10.9 million people annually1. The typhoid toxin is postulated to have a central role in disease pathogenesis, the establishment of chronic infection and human host

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Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.

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