401098

Human IgA, Plasma

• Synonym(s): IgA serum, Plasma IgA
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• assay: ≥95% (SDS-PAGE)
• Quality Level: 200
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgA
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin A (IgA) is the most dominantly generated antibody isotype. They are majorly secreted in the intestinal mucosa. IgA is present in colostrum, serum, saliva, and nasal fluid. It exists in two isotypic forms namely: IgA1 and IgA2. It is generated as secretory IgA (sIgA) and serum IgA. Serum IgA is present in the dimeric form linked by disulfide bond with an additional J chain. sIgA occurs in a monomeric form with two heavy and two light chains.

Application

Human IgA, Plasma has been used in enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Immunoglobulin A (IgA) initiates the alternative pathway of the complement system to elicit protective functions. It facilitates antibody-dependent cell-mediated cytotoxicity (ADCC) by attaching to leukocytes. This interaction boosts microbial destruction. IgA performances the first line of defense in antigen clearance. It avoids proteolysis and hinders the uptake of viral, bacterial, or antigen attachment. It lowers inflammation aroused by the classical pathway complement activation.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 100 mM NaCl, 100 mM Tris-HCl, pH 8.0.

Preparation Note

Prepared from plasma that has been shown by certified tests to be nonreactive for HBsAg and for antibodies to HCV, HBc, HIV-1 and HIV-2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable