328500

ERO1 Inhibitor I, Erodoxin

The ERO1 Inhibitor I, Erodoxin controls the biological activity of ERO1.

• Synonym(s): ERO1 Inhibitor I, Erodoxin, 1-Bromo-5-methoxy-2,4-dinitrobenzene, Endoplasmic Reticulum Oxidase 1 Inhibitor
• Empirical Formula (Hill Notation): C₇H₅BrN₂O₅
• CAS Number: 181995-71-1
• Molecular Weight: 277.03
• MDL number: MFCD00624873
• UNSPSC Code: 12352200
• NACRES: NA.28

Properties

• Quality Level: 100
• assay: ≥99% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: DMSO: 100 mg/mL (Use only fresh DMSO)
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: BrC(C=C(C([N+]([O-])=O)=C1)OC)=C1[N+]([O-])=O

Description

General description

A cell-permeable dinitrobromobenzene compound that acts as a selective inhibitor of yeast Endoplasmic Reticulum Oxidase 1 (ERO1), but has somewhat weaker activity against mouse ERO1α (IC₅₀ = 400 µM). Predicted to form thiol adducts and inhibits ERO1-dependent oxidation of thioredoxin-1 (Trx1) activity in vitro. Shown to cluster with genes involved in protein folding, glycosylation and cell wall biosynthesis, and delay carboxypeptidase Y (CPY) processing to the vacuolar form in wt-yeast cells.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze(-20°C). Stock solutions are stable for up to 1 month at -20°C.

Other Notes

Costanzo, M., et al. 2010. Science327, 425.
Blais, J.D., et al. 2010. J. Biol. Chem.285, 20993.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable