233526

Coagulation Factor Xa, Human Plasma

Coagulation Factor Xa, Human Plasma, CAS 9002-05-5, is a native coagulation factor Xa prepared from Factor X by activation with Russel′s viper venom. The venom is removed after activation.

• Synonym(s): Coagulation Factor Xa, Human Plasma
• CAS Number: 9002-05-5
• Enzyme Commission number: 3.4.21.6 (BRENDA, IUBMB)
• MDL number: MFCD00163610
• UNSPSC Code: 12352202
• NACRES: NA.25

Properties

• Quality Level: 100
• form: lyophilized
• specific activity: ≥170 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: water: soluble
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native coagulation factor Xa from human plasma prepared from homogeneous Factor X by activation with Russel’s viper venom. Hydrolyzes peptide and ester bonds at the carboxyl group of arginine. Specifically catalyzes the activation of prothrombin to thrombin. Prothrombin activation is accelerated by Factor Va, phospholipids, and Ca²⁺. Factor Xa was prepared from homogeneous Factor X, using Russels′ viper venom (RVV), which was removed after activation. Inhibited by AEBSF (Cat. No. 101500), DFP (Cat. No. 30967), PMSF (Cat. No. 52332), and soybean trypsin inhibitor (Cat. No. 650357). Sold on the basis of plasma equivalent units of Factor X. 100 plasma equivalent units are equal to 800 µg of Factor X.

Native coagulation factor Xa from human plasma prepared from homogeneous Factor X by activation with Russel’s viper venom. The venom is removed after activation. During coagulation, in the presence of Ca²⁺ and membrane phospholipids, a complex of Factor Xa and Factor Va (the prothrombinase complex) activates prothrombin to thrombin. Inhibitors include AEBSF (Cat. No. 101500), DFP (Cat. No. 30967), PMSF (Cat. No. 52332), and Soybean Trypsin Inhibitor (Cat. No. 650357). Sold on the basis of plasma equivalent units of Factor X. 100 plasma equivalent units are equal to 800 µg of Factor X.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol CHROMOZYM^® X substrate per min at 25°C.

Physical form

Lyophilized from 700 mM NaCl, 20 mM Tris-HCl, pH 7.4.

Analysis Note

homogeneous by SDS-PAGE

Other Notes

Heeb, M.J., et al. 1994. Proc. Natl. Acad. Sci. USA91, 2728.
Lindhout, T., et al. 1994. Biochem. J.297, 131.
Morishita, H., et al. 1994. Thrombosis Res.73, 193.
Lupu, C., et al. 1993. Thrombosis Haemostasis70, 579.
Jordan, S.P., et al. 1992. Biochemistry31, 5374.
Schoen, P., et al. 1992. Br. J. Haemotol.81, 255.
Tracy, P.B., et al. 1992. Methods Enzymol.215, 329.
Lawson, J.H., and Mann, K.G. 1991. J. Biol. Chem.226, 11317.
Tans, G., et al. 1991. J. Biol. Chem.266, 21864.
Owen, W.G., et al. 1974. J. Biol. Chem.249, 594.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromozym is a registered trademark of Pentapharm AG, Basel

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable