197217

Bcl-x_L BH4₄₋₂₃, Human, Cell-Permeable

A cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity.

• Synonym(s): Bcl-x_L BH4₄₋₂₃, Human, Cell-Permeable, HIV-TAT₄₈₋₅₇-β-Ala-Bcl-x _L BH4₄₋₂₃, Ac-GRKKRRQRRR-βA-SNRELVVDFLSYKLSQKGYS-OH
• Empirical Formula (Hill Notation): C₁₆₅H₂₇₉N₅₉O₄₆
• Molecular Weight: 3825.35
• UNSPSC Code: 12352202

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• solubility: 20% acetic acid: 5 mg/mL; DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Leads to the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨ_m). Contains the conserved N-terminal homology domain (BH4) of Bcl-x_L (amino acids 4 - 23) that has been shown to be essential for inhibiting VDAC activity in liposomes and in isolated mitochondria. The BH4 domain is linked to a carrier peptide, a 10-amino acid HIV-TAT_48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized to the mitochondria.

A highly cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Ultimately results in the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨm). Contains the conserved N-terminal homology domain (BH4) of Bcl-x_L (amino acids 4-23) that has been shown to be essential for inhibiting VDAC activity in liposomes and isolated mitochondria. The BH4 domain is linked to the carrier peptide, the 10-amino acid HIV-TAT_48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized in the mitochondria.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Voltage-dependent anion channel (VDAC)

Product does not compete with ATP.

Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-βAla-Ser-Asn-Arg-Glu-Leu-Val-Val-Asp-Phe-Leu-Ser-Tyr-Lys-Ser-Gln-Lys-Gly-Tyr-Ser-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Chen m., et al. 2002. J. Biol. Chem. 277, 29181.
Futaki, S., et al. 2001. J. Biol. Chem.276, 5836.
Shimizu, S., et al. 2000. Proc. Natl. Acad. Sci. USA97, 3100.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable