MilliporeSigma
All Photos(3)

17-500M

Millipore

Catch and Release® v2.0 Reversible Immunoprecipitation System

Catch & Release v2.0 Reversible Immunoprecipitation System to purify both native & denatured proteins using a variety of antibodies & sample types. Comes in a package of 5 immunoprecipitations.

eCl@ss:
32161000
NACRES:
NB.22

Quality Level

species reactivity (predicted by homology)

all

manufacturer/tradename

Catch And Release®
Upstate®

technique(s)

activity assay: suitable (IP-kinase)
immunoprecipitation (IP): suitable
western blot: suitable

shipped in

wet ice

General description

Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form



Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.

Application

Catch and Release v2.0 should not be used with proteins containing a 6-His tag.

Packaging

50 immunoprecipitations

Components

Catch and Release Wash Buffer, 10X (Cat.# 20-210)

Catch and Release Non-denaturing Elution Buffer, 4X (Cat.# 20-209)

Catch and Release Denaturing Elution Buffer, 1X (Cat.# 20-284)

20-285

20-216

Catch and Release Capture Tubes

Quality

routinely evaluated by immunoprecipitation.

Storage and Stability

All components to be stored at 4°C except the Catch and Release Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.

Legal Information

CATCH AND RELEASE is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Jennifer Altomonte et al.
American journal of physiology. Endocrinology and metabolism, 285(4), E718-E728 (2003-06-05)
Excessive hepatic glucose production is a contributing factor to fasting hyperglycemia in diabetes. Insulin suppresses hepatic glucose production by inhibiting the expression of two gluconeogenic enzymes, phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G-6-Pase). The forkhead transcription factor Foxo1 has been implicated
The expression of p63 is associated with the differential stage in nasopharyngeal carcinoma and EBV infection
Guo, Can, et al
Journal of Translational Medicine, 4, 23-23 (2006)
Blocking of the alpha interferon-induced Jak-Stat signaling pathway by Japanese encephalitis virus infection
Lin, R. J., et al
Journal of virology, 78, 9285-9294 (2004)
Murat Digicaylioglu et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(26), 9855-9860 (2004-06-24)
Erythropoietin (EPO) and insulin-like growth factor I (IGF-I) are cytokines that inhibit neuronal apoptosis. However, their maximal antiapoptotic effect, even at high concentrations, is observed only when neurons are pretreated for several hours before insult. Here we show that simultaneous
Nan Zhang et al.
Diabetes, 53(4), 963-970 (2004-03-30)
Successful islet transplantation depends on the infusion of sufficiently large quantities of islets, of which only approximately 30% become stably engrafted. Rapid and adequate revascularization of transplanted islets is important for islet survival and function. Delayed and insufficient revascularization can

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