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Rap1 Activation Assay Kit
Non-radioactive Rap1 Activation Assay Kit that uses Ral GDS RBD, agarose (Catalog # 14-455) to precipitate Rap1-GTP from cell lysates & detection by a Rap1 specific antibody.
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
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Quality Level
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable
affinity binding assay: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... RAP1GDS1(5910)
Packaging
Kit capacity: 20-30 assays
Components
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Rap1 Activation Lysis Buffer, 2X
Anti-Rap1
Rap1 Assay Reagent (Ral GDS-RBD, agarose) (Cat.# 14-455)
100X GDP, 100mM (Cat.# 20-177)
Rap1 Activation Lysis Buffer, 2X
Anti-Rap1
Rap1 Assay Reagent (Ral GDS-RBD, agarose) (Cat.# 14-455)
Quality
Routinely evaluated by affinity precipitation assay. Ral GDS RBD, agarose precipitated GTP-Rap1 from HEK293 lysates. The precipitated GTP-Rap1 was detected by immunoblot analysis using Anti-Rap1.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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Uma Potla et al.
The Journal of clinical investigation, 124(4), 1757-1769 (2014-03-20)
Injury to the specialized epithelial cells of the glomerulus (podocytes) underlies the pathogenesis of all forms of proteinuric kidney disease; however, the specific genetic changes that mediate podocyte dysfunction after injury are not fully understood. Here, we performed a large-scale
Measurement of GTP-bound Ras-like GTPases by activation-specific probes.
M van Triest et al.
Methods in enzymology, 333, 343-348 (2001-06-13)
B Franke et al.
The EMBO journal, 16(2), 252-259 (1997-01-15)
Rap1 is a small, Ras-like GTPase whose function and regulation are still largely unknown. We have developed a novel assay to monitor the active, GTP-bound form of Rap1 based on the differential affinity of Rap1GTP and Rap1GDP for the Rap
J F Rebhun et al.
The Journal of biological chemistry, 275(45), 34901-34908 (2000-08-10)
Although the Ras subfamily of GTPases consists of approximately 20 members, only a limited number of guanine nucleotide exchange factors (GEFs) that couple extracellular stimuli to Ras protein activation have been identified. Furthermore, no novel downstream effectors have been identified
Rasmus Koefoed Petersen et al.
Molecular and cellular biology, 28(11), 3804-3816 (2008-04-09)
Cyclic AMP (cAMP)-dependent processes are pivotal during the early stages of adipocyte differentiation. We show that exchange protein directly activated by cAMP (Epac), which functions as a guanine nucleotide exchange factor for the Ras-like GTPases Rap1 and Rap2, was required
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