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1.53170

Supelco

Chromolith® Diol (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

Synonym(s):

Chromolith® DIOL 25-4.6 HPLC column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

Chromolith® Diol, L × I.D. 25 mm x 4.6 mm HPLC column

material

PEEK column

Quality Level

100

agency

suitable for USP L20

product line

Chromolith®

feature

endcapped: no

packaging

1 ea of

extent of labeling

11% Carbon loading

parameter

200 bar pressure
45 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

2.5 cm × 4.6 mm

matrix

monolithic silica

matrix active group

Diol bonding phase

pore size

13 nm mesoporosity
2 μm macroporosity

operating pH range

2-7.5

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase

storage temp.

no temp limit

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General description

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure. Chromolith® Diol columns have macropores with 2 µm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å). Chromolith® Diol columns fall under the USP L20 Classification. The column efficiency is comparable to a 5 µm particle size fully porous particulate column. Chromolith® Diol columns are suitable for LC-MS applications.The column dimension of 25 mm length and 4.6 mm I.D. is the best choice for rapid separations on any HPLC System.The revolutionary, monolithic silica with its unique combination of macropores and mesopores enable:

  • Rapid separations at very low column back-pressure
  • High matrix tolerance, allowing time and cost savings with simplified workflows
  • Cost-savings due to extended column lifetimes
  • Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:Chromolith® Diol 5-4.6 guard cartridges (3 pieces) [1.53175] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]Learn more:Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC ColumnsAll Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Monolithic Silica with Bimodal Pore Structure

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Chromolith® HPLC and UHPLC columns

Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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