14-787-M
PI3 Kinase (p110α (H1047R)/p85α) Murine, 10 µg
Complex of N-terminal 6His-tagged, recombinant, full-length mouse p110α containing the mutation H1047R α & untagged, recombinant, full length mouse p85α. For use in Kinase Assays.
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About This Item
UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.32
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biological source
mouse
Quality Level
100
300
recombinant
expressed in baculovirus infected Sf21 cells
mol wt
Mw 129 kDa (p110α; H1047R)
Mw 83.6 kDa (p85α)
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
Gene Information
mouse ... PIK3CA(18706)
General description
Complex of N-terminal 6His-tagged, recombinant, full-length mouse p110α containing the mutation H1047R αand untagged, recombinant, full length mouse p85α.
Product Source: Coexpressed by baculovirus in Sf21 insect cells.
The H1047R substitution in p110α is associated with a number of human cancers. The residue is conserved between the human and mouse sequences. In vitro studies have shown that this mutation confers increased lipid kinase activity compared to wild type, and is able to induce oncogenic transformation.
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
Biochem/physiol Actions
Protein Target: PI3Kα/p85α
Target Sub-Family: PI3K/PI4K
Packaging
Also available in 250μg size --call for pricing and availability and reference catalog number 14-787M when ordering the 250μg size.
Physical form
Ni2+/NTA-agarose
Storage and Stability
Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
High frequency of mutations of the PIK3CA gene in human cancers.
Yardena Samuels et al.
Science (New York, N.Y.), 304(5670), 554-554 (2004-03-16)
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