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1.16141

Supelco

Sucrose (Saccharose) Test

reflectometric, 0.25-2.50 g/L (sucrose), for use with REFLECTOQUANT®

Quality Level

description

quantitative

analyte chemical class(es)

sugars

reaction suitability

reaction type: enzymatic reaction

measuring range

0.25-2.50 g/L (sucrose)

compatibility

RQflex® 10 Plus
RQflex® 10
RQflex® 20
for use with REFLECTOQUANT®

detection method

reflectometric

storage temp.

2-8°C

General description

The Reflectoquant Sucrose test is suitable for determination of sucrose in beverages, e. g. fruit/vegetable juice and refreshment drinks after appropriate sample pretreatment or dilution. Sucrose (saccharose) is cleaved by sucrose phosphorylase into fructose and glucose-1-phosphate. The latter is converted by phosphoglucomutase into glucose-6-phosphate. This is in turn oxidized by NAD under the catalytic effect of glucose-6-phosphate dehydrogenase to gluconate-6-phosphate. In the presence of diaphorase the NADH formed in the process reduces a tetrazolium salt to a blue formazan that is determined reflectometrically on the RQflex. For the determination of only glucose please refer to our MQuant or Reflectoquant Glucose tests.
Method: reflectometric with test strips and reagent 0.25 - 2.50 g/l Reflectoquant®

Linkage

Replaces: 1161410007; 1.16141.0007

Analysis Note

function control: entspricht
Assay
- standard 1:
0.26 - 0.34 mg/l
- standard 2: 1.12 - 1.38 mg/l
- standard 3: 1.80 - 2.20 mg/l

Legal Information

REFLECTOQUANT is a registered trademark of Merck KGaA, Darmstadt, Germany
RQFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10-13 - German Storage Class 10 to 13

Certificate of Analysis

Certificate of Origin

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