1.11608

Ethidium bromide

(1% solution in water) for electrophoresis

• UNSPSC Code: 41105305

Properties

• Quality Level: 100
• form: liquid
• pH: 4.4 (20 °C in H₂O)
• density: 1.00 g/cm³ at 20 °C
• storage temp.: 2-8°C

Description

Application

Ethidium bromide has been used to stain polyacrylamide gels for small-scale transcription reactions. It has also been used for the quantification of DNA.

Biochem/physiol Actions

Ethidium bromide is a red fluorescent dye that reacts with the DNA of membrane-damaged cells by intercalating. It is used for visualizing DNA in gels. Ethidium bromide is carcinogenic with moderate toxicity.

Features and Benefits

Robust and easy to use

Analysis Note

Identity (UV/VIS-Spectrum): passes test
Absorption maximum (water): 478 - 482 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.004 %; water): 140 - 160
Suitability for electrophoresis: passes test

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H331 - H341
• pcodes: P202 - P261 - P271 - P280 - P304 + P340 + P311 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Muta. 2
• Storage Class: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable