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MilliporeSigma
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Key Documents

1.10328

Millipore

Blood agar (base) no. 2

for the cultivation of fastidious microorganisms

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About This Item

UNSPSC Code:
41106213

Quality Level

form

medium granules (dehydrated (DCM))

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

7.2-7.6 (25 °C, 40 g/L in H2O, after autoclaving)

solubility

40 g/L

application(s)

clinical
microbiology

storage temp.

15-25°C

suitability

aerobic bacteria

General description

for the cultivation of fastidious microorganisms

Analysis Note

Appearance (clearness)clearAppearance (colour)yellowish brownpH-value (25 °C)7.2 - 7.6Gel strength (Gelomat assay) (Base)≥ 50 gGel strength (Gelomat assay) (with blood)≥ 40 gStability test (Colour and hemolysis)passes testRecovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))≥ 70 %Recovery on test medium (Streptococcus pyogenes ATCC 19615)≥ 70 %Recovery on test medium (Streptococcus pneumoniae ATCC 6305)≥ 70 %Recovery on test medium (Streptococcus agalactiae ATCC 13813)≥ 70 %Recovery on test medium (Listeria monocytogenes ATCC 19118)≥ 70 %Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001))≥ 70 %Recovery on test medium (Clostridium perfringens ATCC 13124 (WDCM 00007))≥ 70 %Hemolysis (Streptococcus pyogenes ATCC 19615)βHemolysis (Streptococcus pneumoniae ATCC 6305)αHemolysis (Streptococcus agalactiae ATCC 13813)-Hemolysis (Bacillus cereus ATCC 11778 (WDCM 00001))βHemolysis (Clostridium perfringens ATCC 13124 (WDCM 00007))βBacitracin test (Streptococcus pyogenes ATCC 19615)+Incubation: 24 hrs.; 35 °C; aerobic (Clostridium perfringens anaerobic with Anaerocult A).

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

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Protocols

In recent years it has been recognized that Listeria is an important public health problem. The disease affects primarily people of advanced age, pregnant women, newborns, and adults with weakened immune systems.

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