1.09684

Formamide

for analysis EMSURE^®

• Synonym(s): Formic acid amide, Methane amide, Amide C₁, Formic amide
• Linear Formula: HCONH₂
• CAS Number: 75-12-7
• Molecular Weight: 45.04
• Beilstein/REAXYS Number: 505995
• MDL number: MFCD00007941
• UNSPSC Code: 12191502
• EC Index Number: 200-842-0
• NACRES: NB.21

Properties

• vapor density: 1.55 (vs air)
• Quality Level: 300
• vapor pressure: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• product line: EMSURE^®
• assay: ≥99.2% (N)
• form: liquid
• autoignition temp.: 932 °F
• potency: 5325 mg/kg LD₅₀, oral (Rat); 17000 mg/kg LD₅₀, skin (Rabbit)
• expl. lim.: 2.7-19.0 % (v/v)
• technique(s): hybridization: suitable
• impurities: ≤0.005% Sulfated ash (600 °C); ≤0.02% Formic acid; ≤0.1% Ammonium Formate; ≤0.1% Water (Karl Fischer); ≤1 ppm Cd (Cadmium); ≤1 ppm Chloride (Cl); ≤1 ppm Cu (Copper)
• color: clear; APHA: ≤10
• refractive index: n20/D 1.447 (lit.)
• pH: 8-10 (20 °C, 200 g/L in H₂O)
• bp: 210 °C (lit.)
• mp: 2-3 °C (lit.)
• transition temp: flash point 175 °C
• density: 1.134 g/mL at 25 °C (lit.)
• cation traces: Fe: ≤1 ppm; Pb: ≤1 ppm; Zn: ≤1 ppm
• storage temp.: 15-25°C
• SMILES string: NC=O
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• InChI key: ZHNUHDYFZUAESO-UHFFFAOYSA-N

Description

General description

EMSURE grade solvents are suitable for a broad spectrum of classical lab applications, and are frequently used in regulated and highly demanding lab applications. EMSURE provides worldwide best and most extensive product specifications. We declare our EMSURE range to be in compliance with the ACS, with the reagent part of the European Pharmacopoeia (Reag. Ph Eur) and also with the ISO standards.

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Analysis Note

Assay (N; Kjeldahl): ≥ 99.2 %
Identity (IR): passes test
Appearance: Clear, colorless liquid.
Colour (APHA): ≤ 10 APHA
Density (d 20 °C/ 4 °C): 1.132 - 1.135
Solidification point: 2.0 - 3.0 °C
Boiling point (2 kPa): about 103
Chloride (Cl): ≤ 1 ppm
Cd (Cadmium): ≤ 1 ppm
Cu (Copper): ≤ 1 ppm
Fe (Iron): ≤ 1 ppm
Pb (Lead): ≤ 1 ppm
Zn (Zinc): ≤ 1 ppm
Formic acid: ≤ 0.02 %
Ammonium Formate: ≤ 0.1 %
Sulfated ash (600 °C): ≤ 0.005 %
Water (according to Karl Fischer): ≤ 0.1 %

Legal Information

EMSURE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H351 - H360D - H373
• pcodes: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• target_organs: Blood
• Storage Class: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 1
• flash_point_f: 305.6 °F
• flash_point_c: 152 °C