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1.05642

Supelco

HPTLC Silica gel 60 F₂₅₄

pkg of 50 plates, plate L × W 20 cm × 10 cm, glass support

material

glass support
silica gel 60 matrix

Quality Level

packaging

pkg of 50 plates

application(s)

thin layer chromatography (TLC): suitable

layer thickness

200 μm

plate L × W

20 cm × 10 cm

storage temp.

2-30°C

General description

50 Glass plates 20 x 10 cm
HPTLC plates deliver fast and quantitative analysis of complex samples for manual or automated use. Our HPTLC silica plates work three times faster than conventional TLC plates – and they are more sensitive. The particle sizes range in HPTLC is between 4-8 µm, which creates a smoother surface and a higher separation power than TLC plates.

Application

Glass HPTLC Silica gel 60 F₂₅₄ plates. Silica gel HPTLC plates size 20 x 10 cm, 50 sheets.

Linkage

Replaces: 5642-6; 5642

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)

Certificate of Analysis

Certificate of Origin

Articles

High-Performance Thin-Layer Chromatography: A Fast and Efficient Fingerprint Analysis Method for Medicinal Plants

In this article we present several HPTLC applications and analytical standards for ginsenosides.

Separation of Steviol Glycosides by HPTLC: Introducing a New Stevia Extract Reference Material

HPTLC is an efficient method to determine Steviol Glycosides content in Stevia using our HPTLC plates and reference materials, including a new introduced Stevia extract reference material.

Protocols

quantification of caffeine

HPTLC was used to analyze caffeine in coffee. Filtration of coffee through a PTFE syringe filter was the only sample preparation. Quantitation was conducted with a TLC scanner.

Chromatographic Testing of Black Pepper According to the United States Pharmacopeia

Powdered black pepper was chosen as example for dietary supplements, being a source for pierine. It is tested according to the current United States Pharmacopeia (USP) guidelines using HPTLC and HPLC.

Sildenafil Citrate USP Method Using HPTLC Plates for the Limit of Imidazole Test

In the present study we demonstrate compliance of silica gel 60 F254 HPTLC plates with USP requirements, and how HPTLC plates are suitable for the Sildenafil citrate-Limit of Imidazole test.

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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