1.04171

Nuclear fast red (C.I. 60760)

• Empirical Formula (Hill Notation): C₁₄H₈NNaO₇S
• CAS Number: 6409-77-4
• Molecular Weight: 357.27
• MDL number: MFCD00078493
• NACRES: NA.21

Properties

• form: solid
• Quality Level: 200
• composition: Dye, ≥90% spectrophotometric assay
• pH: 5.5-7.0 (20 °C, 1 g/L in H₂O)
• solubility: 1 g/L
• bulk density: 400‑450 kg/m³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1
• InChI key: IFSXZLJQEKGQAF-UHFFFAOYSA-M

Description

General description

Nuclear fast red (C.I. 60760) for microscopy Certistain^®, is a dry dye for microscopy that is often used for staining nuclei in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of a Nuclear fast red staining solution. It is commonly used for counterstaining in histochemical reactions, e.g. in Berlin blue staining for detection of iron or in silver plating of reticulin fibers, as well as nuclear staining with Alcian blue. As the dye belongs to the portfolio of Certistain^® dyes, it is chemically analyzed according to strict specifications before release and a high staining quality, with reliable staining results is guaranteed.

Application

for microscopy Certistain^®

Analysis Note

Dye content (spectrophotometrically): ≥ 90 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ 1 (Dimethyl sulfoxide): 557 - 559 nm
Absorption maximum λ 2 (Dimethyl sulfoxide): 520 - 522 nm
Spec. Absorptivity A 1%/1cm
- λ1_max; 0.02 g/l; dimethyl sulphoxide:
380 - 422
- λλ2_max; 0.02 g/l; Dimethyl sulphoxide: 383 - 426
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable