07-261-I

Anti-mGluR2 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): 07-261, Histone H2AX, H2a/x, Histone H2A.X
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat
• species reactivity (predicted by homology): mouse (based on 100% sequence homology)
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_001153825
• UniProt accession no.: Q14BI2
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Grm2(108068) ; rat ... Grm2(24415)

Description

General description

The metabotropic glutamate receptors belong to a family of G protein-coupled receptors that have been divided into 3 groups based on their sequence homology, putative signal transduction mechanisms, and pharmacologic properties. mGluR2 belongs to the Group II. It is widely expressed in different regions of fetal and the adult brain. Ligand binding to GluR2 induces a conformation change in the receptor, which triggers signaling to modulate the activity of down-stream effectors, mainly via the inhibition of adenylate cyclase activity. Activation of mGluR2 leads to inhibition of glutamate release at the synaptic junction

Immunogen

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of mouse mGlur2.

Application

Anti-mGluR2 Antibody is an antibody against mGluR2 for use in Western Blotting, Immunoprecipitation.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated mGlur2 in 500 µg of rat brain microsomal prep.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in rat brain microsomal prep.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected mGlur2 in 10 µg of rat brain microsomal prep.

Target description

~95/200 kDa observed. The lower band corresponds to the calculated molecular weight of mGluR2 (~100 kDa) and the upper band is likely a receptor dimer and frequently appears in immunoblots of mGluRs (Reid and Romano, 2001; Romano et al., 1996).

Linkage

Replaces: 07-261

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable