07-2189

Anti-CAPZIP Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): RCSD domain containing 1, capZ-interacting protein, Protein kinase substrate CapZIP, protein kinase substrate MK2S4, RCSD domain-containing protein 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• Quality Level: 100
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_443094
• UniProt accession no.: Q6JBY9
• shipped in: wet ice
• Gene Information: human ... RCSD1(92241)

Description

General description

CAPZIP (CapZ-interacting protein) is highly expressed in immune cells and muscle and uniquely interacts with CapZ, an F-actin capping protein, in splenocytes. Phosphorylation of CAPZIP is thought to regulate CapZ ability to remodel actin filament assembly. CAPZIP may also contain a CPI (capping protein interaction) motif that seems to restrict capping protein to a low-affinity, filament-binding conformation.

Immunogen

GST-tagged recombinant protein corresponding to human CAPZIP.

Application

Anti-CAPZIP detects levels of CAPZIP proteins & has been published & validated for use in WB & IP.

Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated CAPZIP from Jurkat cell lysate.

Other homologies: Mouse (64% sequence homology). Rat (58% sequence homology).

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Quality

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 1:250 dilution of this antibody detected CAPZIP on 10 µg of Jurkat cell lysate.

Target description

~70 kDa observed. The native form of CAPZIP runs anomalously, with a molecular mass of 70 kDa (much slower than its real molecular mass). The 47 kDa band may be non-specific, a splice variant or a degradation product. (Eyers, C.E., et al. (2005). Biochem J. 389(1):127-135.). An uncharacterized band appears at ~18 kDa

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Jurkat cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable