07-1238
Anti-phospho-MARCKS (Ser152/156) Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate, 80 kDa protein, light chain
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer152/pSer156)
Gene Information
human ... MARCKS(4082)
General description
Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a widely expressed substrate of PKC. MARCKS is essential in neuronal development, as mice deficient in it die shortly after birth. Through its basic effector region, MARCKS associates with acidic membrane phospholipids, actin filaments, and calmodulin. Poly (ADP-ribose) also binds the basic effector region, blocking calmodulin binding, membrane association, and phosphorylation by PKC, suggesting that MARCKS may be an effector of the DNA-damage signal transduced by poly (ADP-ribose).
Specificity
MARCKS, phosphoSer152/156.
Immunogen
Epitope: phosphoSer 152/156
Synthetic peptide corresponding to amino acids surrounding the phosphoSer152/156 of rat MARCKS.
Application
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining of MARCKS With Citrate pH 6.0 Epitope Retrieval: Rat Placenta
Optimal working dilutions must be determined by the end user.
Representative images from a previous lot.
Optimal Staining of MARCKS With Citrate pH 6.0 Epitope Retrieval: Rat Placenta
Optimal working dilutions must be determined by the end user.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
This Anti-phospho-MARCKS (Ser152/156) Antibody is validated for use in WB, IH(P) for the detection of phospho-MARCKS (Ser152/156).
Quality
Routinely evaluated by Western Blot on EGF treated A431 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phosphorylated MARCKS on 10 μg of EGF treated A431 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phosphorylated MARCKS on 10 μg of EGF treated A431 lysates.
Target description
~80 kDa
Linkage
Replaces: AB5879
Physical form
Affinity purified
Purified rabbit polyclonal IgG in 10 mM Hepes, pH 7.5, with 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Storage and Stability
Stable at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
A431 Cell Lysate
A431 Cell Lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificates of Analysis (COA)
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Chien-Neng Wang et al.
British journal of pharmacology, 176(8), 1122-1134 (2019-02-02)
Myristoylated alanine-rich C kinase substrate (MARCKS), a PKC substrate, facilitates mucus production and neutrophil migration. However, the effects of therapeutic procedures targeting the phosphorylation site of MARCKS on steroid-resistant asthma and the mechanisms underlying such effects have not yet been
Dan Yu et al.
Journal of the American Heart Association, 4(10), e002255-e002255 (2015-10-10)
Transcription of the myristoylated alanine-rich C kinase substrate (MARCKS) is upregulated in animal models of intimal hyperplasia. MARCKS knockdown inhibits vascular smooth muscle cell (VSMC) migration in vitro; however, the mechanism is as yet unknown. We sought to elucidate the
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