06-946

Anti-hyperacetylated Histone H4 (Penta) Antibody

serum, Upstate^®

• Synonym(s): H4 histone family, member A, histone 1, H4a, histone cluster 1, H4a
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, eukaryotes, Tetrahymena sp.
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_778224
• UniProt accession no.: P62805
• shipped in: wet ice
• target post-translational modification: acetylation (Penta-acetylation)
• Gene Information: human ... H4C1(8359)

Description

General description

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Specificity

Expected to cross-react with Tetrahymena and Eukaryote.

Other species not tested.

Recognizes hyperacetylated histone H4, Mr 10 kDa (SDS-PAGE); tri- and tetra-acetylated histone H4 on acid-urea gels. May cross-react with other acetylated proteins.

Immunogen

KLH-conjugated, synthetic peptide
(AGGAcKGGAcKGGAcKGGAcKGGAcKGGC) related to amino acids 2 to 19 of Tetrahymena thermophila histone H4.

Application

Chromatin Immunoprecipitation:
Reported to immunoprecipitate transcriptionally active chromatin containing acetylated histone H4. (Perry, C.A., 1993)

Immunocytochemistry:
Reported to exclusively stain Tetrahymena macronuclei1 and chromatin in HeLa nuclei. (Perry, C.A., 1993)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-hyperacetylated Histone H4 (Penta) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, ICC, WB to detect hyperacetylated Histone H4 (Penta) also known as H4 histone family member A.

Quality

Routinely evaluated by Western Blot on HepG2 lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected HYPERACETYLATED HIS H4 on 10 μg of HepG2 lysates.

Target description

Approx. 10 kDa

Physical form

Rabbit polyclonal antiserum IgG in buffer containing 30% glycerol.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Tetrahymena cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.