06-1352-I

Anti-acetyl Histone H4 (Lys12) Antibody

0.5 mg/mL, from rabbit

• Synonym(s): Histone H4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, mouse, human
• concentration: 0.5 mg/mL
• technique(s): dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_003529
• UniProt accession no.: P62805
• shipped in: wet ice
• target post-translational modification: acetylation (Lys12)
• Gene Information: human ... HIST2H4B(554313)

Description

General description

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The histones have an amino terminal tail, a globular domain, and a carboxy-terminal tail. The four core histones, H2A, H2B, H3 and H4 assemble into the octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped in a sequence-independent manner around the octamer, forming the basic subunit of chromatin, the nucleosome. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4 acetylated at Lys12.

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected acetyl Histone H4 (Lys12) in HeLa cells.

Dot Blot (Specificity) Analysis: 0.1 µg/mL from a representative lot detected acetyl Histone H4 (Lys12) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and in an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

This Anti-acetyl Histone H4 (Lys12) Antibody is validated for use in western blotting, ICC & dot blot for the detection of acetyl Histone H4 (Lys12).

Quality

Evaluated by Western Blotting in sodium butyrate treated HeLa acid extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected acetyl Histone H4 (Lys12) in 10 µg of sodium butyrate treated HeLa acid extract.

Target description

~13 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable