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05-412

Sigma-Aldrich

Anti-GSK3 Antibody, clone 4G-1E

clone 4G-1E, Upstate®, from mouse

Synonym(s):

GSK-3 alpha, glycogen synthase kinase 3 alpha

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4G-1E, monoclonal

species reactivity

mouse, Dictyostelium sp., Schizosaccharomyces pombe, rat, vertebrates, human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1

suitability

not suitable for immunoprecipitation

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GSK3A(2931)
mouse ... Gsk3A(606496)
rat ... Gsk3A(50686)

General description

Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways. Two isoforms of GSK-3 are reported in mammals: a 51 kDa GSK-3a and a 47 kDa GSK-3b. These two isoforms exhibit about 98% homology in their kinase domains, but share only about 36% identity in the last 76 C-terminal amino acid residues. GSK-3a contains a glycine-rich extension at its N-terminus. A minor (~15% of total) splice variant of GSK-3b, GSK-3b2, has also been identified, which contains a 13-residue insert within the kinase domain. It exhibits reduced kinase activity towards tau protein compared with ‘unspliced’ GSK-3b. GSK-3b2 is localized primarily to neuronal cell bodies, unlike unspliced GSK-3b that is also found in neuronal processes. GSK-3b plays a key inhibitory role in the Wnt signaling pathway. Wnt genes encode a large family of secreted, cysteine-rich proteins that are important in development and in maintenance of adult tissues. Abnormalities in Wnt signaling are reported to promote both human dgenerative diseases and cancer.

Specificity

Because of the extremely high conservation of this immunogen among the known enzymes of many different species, this antibody is predicted to cross-react with nearly all species homologues.
GSK3, α and β isoforms

Immunogen

18 residue synthetic peptide (C-KQLLHGEP NVSYICSRY) corresponding to amino acids 203-219 derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK3/shaggy enzyme, coupled to ovalbumin. Clone 4G-1E.

Application

Anti-GSK3 Antibody, clone 4G-1E detects level of GSK3 & has been published & validated for use in IC & WB.
Immunoprecipitation:
Not recommended.

Immunocytochemistry:
10 µg/mL of a previous lot showed positive immunostaining for GSK3 in L6 cells fixed with acetone.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

Quality

Routinely evaluated by immunoblot on human Jurkat cells, human A431, rat L6, rat PC12 or mouse 3T3 cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected GSK3 in RIPA lysates from human Jurkat cells; previously GSK3 was detected in RIPA lysates from human A431, rat L6 and PC12, and mouse 3T3 cells.

Target description

51 kDa (α) or 46 kDa (β)

Physical form

Format: Purified
Protein G Purified
Protein G purified mouse IgG1 in storage buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Regulation of neural migration by the CREB/CREM transcription factors and altered Dab1 levels in CREB/CREM mutants.
Carmen Diaz-Ruiz,Rosanna Parlato,Fernando Aguado,Jesus M Ure?a,Ferran Burgaya et al.
Molecular and Cellular Neurosciences null
Assays for glycogen synthase kinase-3 (GSK-3).
D Cross
Methods in molecular biology (Clifton, N.J.), 124, 147-159 (2000-12-02)
Glycogen synthase kinase 3 regulates PAX3-FKHR-mediated cell proliferation in human alveolar rhabdomyosarcoma cells.
Fu-Yue Zeng,Hanqing Dong,Jimmy Cui,Lingling Liu,Taosheng Chen
Biochemical and biophysical research communications null
Divergent cell signaling after short-term intensified endurance training in human skeletal muscle.
Benziane, B; Burton, TJ; Scanlan, B; Galuska, D; Canny, BJ; Chibalin, AV; Zierath, JR; Stepto, NK
American Journal of Physiology. Endocrinology and Metabolism null
Chronic renin inhibition with aliskiren improves glucose tolerance, insulin sensitivity, and skeletal muscle glucose transport activity in obese Zucker rats.
Marchionne, EM; Diamond-Stanic, MK; Prasonnarong, M; Henriksen, EJ
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology null

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