05-369X-555

Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor^™ 555

clone C464.6, Upstate^®, from mouse

• Synonym(s): Anti-CMT2DD
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 555
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: C464.6, monoclonal
• species reactivity: mouse, human, rabbit, Xenopus, sheep, monkey, canine, pig, rat
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Upstate^®
• technique(s): flow cytometry: suitable; immunofluorescence: suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_152296.3
• UniProt accession no.: Q9N0Z6
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ATP1A3(478)

Description

Specificity

Specific for the α-1 subunit isoform.

Immunogen

Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.

Application

Detect Na+/K+ ATPase α-1 using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor 555 validated for use in FC, IF & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Quality

Immunoblot Analysis: 2µg/ml of this lot detected α-1 subunit in 40μg of rat brain microsomal preparation.

Immunocytochemistry: 2µg/ml of this lot showed positive immunostaining for α-1 subunit in Colo 205 cells.

Flow Cytometry: 0.2μg of this lot detected α-1 subunit in fixed NCI H292 cells (see data).

Target description

113 kDa

Physical form

25µg Alexa Fluor^™ 555-conjugated protein G purified mouse IgG1κ in 100µl of PBS containing 1% BSA, 0.05% Tween-20, 0.05% sodium azide. Liquid at 4°C.

Protein G Purified

Storage and Stability

Do Not Freeze. Do not store the material diluted. Stable for 1 year at 4°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing cap.

Analysis Note

Control
Included Negative Control: Catalog # 16-239, Alexa Fluor^™ 555-conjugated Normal Mouse IgG.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Alexa Fluor^™ is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable