L040150

Cap 1

• Synonym(s): Cap A
• UNSPSC Code: 12352106
• NACRES: NA.21

Properties

• type: for DNA synthesis
• Quality Level: 200
• product line: Proligo Reagents
• form: liquid
• storage condition: dry at room temperature
• technique(s): DNA synthesis: suitable
• impurities: ≤200 ppm water content (Karl Fischer)
• color: clear
• suitability: conforms to structure (GC, Formulation)
• storage temp.: room temp

Description

General description

Cap 1 solution consists of n-methylimidazole, lutidine, and acetonitrile (ACN).

Application

Cap 1 is suitable for use after the phosphoramidite reaction/coupling step.

Biochem/physiol Actions

Cap 1 solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Preparation Note

Acetonitrile / Lutidine / N-Methylimidazole 8/1/1 (v/v/v)

Safety Information

• pictograms: GHS02,GHS08,GHS05,GHS07
• signalword: Danger
• hcodes: H225,H302 + H312 + H332,H314,H361d
• pcodes: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Repr. 2 - Skin Corr. 1B
• Storage Class: 3 - Flammable liquids
• wgk_germany: WGK 3
• flash_point_f: 35.6 °F - closed cup
• flash_point_c: 2.0 °C - closed cup