GEN-7003

Cationic Liposomes for DNA/RNA delivery

DOTAP:Cholesterol (1:1) containing 0.5% NBD-DOPE (Fluorescent)

• UNSPSC Code: 12352211
• NACRES: NA.25

Properties

• Quality Level: 100
• contains: NBD-DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-; 1,3-benzoxadiazol-4-yl)) as fluorescent label
• composition: Deionized RNAse-free Water
• concentration: 0.02 mM (NBD-DOPE); 2 mM (Cholesterol); 2 mM (DOTAP)
• impurities: 1:1 mol/mol DOTAP:Cholesterol
• particle size: 100 nm
• pH: 7
• absorbance ratio: _{460/535 nm}

Description

General description

Cationic liposomes are traditionally used for the delivery of genetic materials such as various types of DNA (pDNA, cDNA, CpG DNA, oligonucleotide, antisense oligonucleotide), various types of RNA such as (siRNA, mRNA) and nucleic acid mimics (NAMs). The positive, cationic charge of the lipids is used to neutralize the negative charge of nucleic acids and results in greater encapsulation efficiency, cellular uptake and endosomal delivery.

Application

Drug delivery
Gene delivery
Lipid-protein interactions

Storage and Stability

Liposomes should never be frozen. Liposomes should be stored in the dark at 4°C, except when brought to room temperature for brief periods prior to use.

Liposomes are made under sterile conditions. If you need to take multiple aliquots out of the vial, it is advised to take extreme care in not contaminating the vial. It is recommended to handle the vial under a sterile hood to maintain the sterility of the product. Liposomes should never be frozen. Ice crystals that form during freezing will rupture the lipid membrane of the liposomes and change the size of liposomes particles.

Legal Information

Genesome is a trademark of Encapsula NanoSciences

Product of Encapsula Nanosciences

Disclaimer

For research use only

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable