927821
EBX2-alkyne
≥95%
Synonym(s):
5-Methyl-1-((trimethylsilyl)ethynyl)-1l3-benzo[d][1,2]iodaoxol-3(1H)-one
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About This Item
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Quality Level
assay
≥95%
form
powder
storage temp.
−20°C
SMILES string
CC1=CC(C(OI2C#C[Si](C)(C)C)=O)=C2C=C1
Application
EBX2-alkyne is a hypervalent iodine reagent used for labeling cysteines. A method was developed using cysteine-reactive compounds including this one to allow for unbiased analysis of proteomic data in quantitative applications . The method uses light or heavy labeling with the isotopically labelled desthiobiotin azide (isoDTB) tag for mass spectrometry analysis . Analysis then uses the isotopic tandem orthogonal proteolysis activity-based protein profiling (isoTOP-ABPP) workflow .
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Profiling the proteome-wide selectivity of diverse electrophiles.
ChemRxiv : the preprint server for chemistry (2021)
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Nature, 468(7325), 790-795 (2010-11-19)
Cysteine is the most intrinsically nucleophilic amino acid in proteins, where its reactivity is tuned to perform diverse biochemical functions. The absence of a consensus sequence that defines functional cysteines in proteins has hindered their discovery and characterization. Here we
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